Comparison of Three Different Methods for Detection of Il28 Rs12979860 Polymorphisms As a Predictor of Treatment Outcome in Patients With Hepatitis C Virus Publisher



Fateh A^{1, 2} ; Aghasadeghi M³ ; Siadat SD¹ ; Vaziri F¹ ; Sadeghi F⁴ ; Fateh R⁵ ; Keyvani H⁶ ; Tasbiti AH¹ ; Yari S¹ ; Ataeipirkooh A⁶ ; Monavari SH⁶ Show Affiliations
Source: Osong Public Health and Research Perspectives Published:2016

Abstract

Objectives: This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C. Methods: A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR. Results: The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710). Conclusion: Screening by ARMS - PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice. © 2016.