L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity

L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity Publisher Pubmed

Monajati M^{1, 2} ; Tamaddon AM^{1, 2, 3} ; Abolmaali SS^{1, 2} ; Yousefi G^{2, 3} ; Javanmardi S² ; Borandeh S² ; Heidari R⁴ ; Azarpira N⁵ ; Dinarvand R⁶

Show Affiliations

1. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
2. Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz, Iran
3. Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
4. Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
5. Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
6. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417614315, Iran

Source: Colloids and Surfaces B: Biointerfaces Published:2023

Abstract

L-asparaginase (ASNase) enzyme has limited therapeutic use due to its poor pharmacokinetics and immunogenicity. To overcome these obstacles, we immobilized ASNase in biocompatible poly hydroxypropyl methacrylamide (P(HPMA))-based nanogels simply formed through the host-guest inclusion complex of ASNase-conjugated random copolymer of HPMA and polyethylene glycol (PEG) acrylate (P(HPMA-MPEGA)) and α-cyclodextrin dimer (bisCD) using cystamine as a linker. The effects of bisCD and polymer concentrations on particle size, gelation time, and recovery of enzyme activity were investigated. The ASNase-conjugated bisCD nanogels were discrete, homogeneous, and spherical with a mean projected diameter of 148 ± 41 nm. ASNase immobilized in the bisCD nanogels caused cytotoxicity on HL-60 cell line with IC50 of 3 IU/ml. In-vivo rat study revealed that the immobilized ASNase reduced the enzyme antigenicity and resulted in 8.1 folds longer circulation half-life than the native enzyme. Conclusively, immobilization of ASNase in P(HPMA-MPEGA) and bisCD supramolecular nanogels could enhance the therapeutic value of ASNase in cancer chemotherapy. © 2023

2. Enhanced L-Asparaginase Stability Through Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma With Bis(Α-Cyclodextrin), Biochemical Engineering Journal (2023)

3. Applications of Raft Polymerization for Chemical and Enzymatic Stabilization of L-Asparaginase Conjugates With Well-Defined Poly(Hpma), New Journal of Chemistry (2019)

4. 3D Printing of Self-Healing Materials for Drug Delivery Applications: Promises, Advances and Outlooks, Bioprinting (2024)

5. Immobilization of L-Asparaginase on Aspartic Acid Functionalized Graphene Oxide Nanosheet: Enzyme Kinetics and Stability Studies, Chemical Engineering Journal (2018)

Experts (# of related papers)

Rassoul Dinarvand (3)

Style	Citing Format
MLA	Monajati M, et al.. "L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity." Colloids and Surfaces B: Biointerfaces, vol. 225, no. , 2023, pp. -.
APA	Monajati M, Tamaddon AM, Abolmaali SS, Yousefi G, Javanmardi S, Borandeh S, Heidari R, Azarpira N, Dinarvand R (2023). L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity. Colloids and Surfaces B: Biointerfaces, 225(), -.
Chicago	Monajati M, Tamaddon AM, Abolmaali SS, Yousefi G, Javanmardi S, Borandeh S, Heidari R, Azarpira N, Dinarvand R. "L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity." Colloids and Surfaces B: Biointerfaces 225, no. (2023): -.
Harvard	Monajati M et al. (2023) 'L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity', Colloids and Surfaces B: Biointerfaces, 225(), pp. -.
Vancouver	Monajati M, Tamaddon AM, Abolmaali SS, Yousefi G, Javanmardi S, Borandeh S, et al.. L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity. Colloids and Surfaces B: Biointerfaces. 2023;225():-.
BibTex	@article{ author = {Monajati M and Tamaddon AM and Abolmaali SS and Yousefi G and Javanmardi S and Borandeh S and Heidari R and Azarpira N and Dinarvand R}, title = {L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity}, journal = {Colloids and Surfaces B: Biointerfaces}, volume = {225}, number = {}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Monajati M AU - Tamaddon AM AU - Abolmaali SS AU - Yousefi G AU - Javanmardi S AU - Borandeh S AU - Heidari R AU - Azarpira N AU - Dinarvand R TI - L-Asparaginase Immobilization in Supramolecular Nanogels of Peg-Grafted Poly Hpma and Bis(Α-Cyclodextrin) to Enhance Pharmacokinetics and Lower Enzyme Antigenicity JO - Colloids and Surfaces B: Biointerfaces VL - 225 IS - SP - EP - PY - 2023 ER -

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