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Standardized Gmp-Compliant Scalable 3D-Bioprocessing of Epidermal Stem Cells for Diabetic Foot Ulcers Publisher Pubmed



H Adibi HOSSEIN ; B Arjmand BABAK ; Hr Aghayan Hamid REZA ; E Bahramivahdat ELAHE ; S Alavimoghadam SEPIDEH ; M Rezaeitavirani MOSTAFA ; R Arjmand RASTA ; N Namazi NAZLI ; Ba Larijani Bagher A
Authors

Source: Methods in Molecular Biology Published:2024


Abstract

Diabetic foot ulcers (DFUs) pose a significant threat to the health and well-being of individuals with diabetes, often leading to lower limb amputations. Fortunately, epidermal stem cell therapy offers hope for improving the treatment of DFUs. By leveraging 3D culture techniques, the scalability of stem cell manufacturing can be greatly enhanced. In particular, using bioactive materials and scaffolds can promote the healing potential of cells, enhance their proliferation, and facilitate their survival. Furthermore, 3D tissue-mimicking cultures can accurately replicate the complex interactions between cells and extracellular matrix, thereby ensuring that the stem cells are primed for therapeutic application. To ensure the safety and quality of these stem cells, it is essential to adhere to good manufacturing practice (GMP) principles during cultivation. This chapter provides a comprehensive overview of the step-by-step process for GMP-based 3D epidermal stem cell cultivation, thus laying the groundwork for developing reliable regenerative medicine therapies. © 2024 Elsevier B.V., All rights reserved.
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