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A Novel Silk/Pes Hybrid Nanofibrous Scaffold Promotes the in Vitro Proliferation and Differentiation of Adipose-Derived Mesenchymal Stem Cells Into Insulin Producing Cells Publisher



Hosseini M1 ; Dadashinoshahr K2 ; Islami M3 ; Saburi E4 ; Nikpoor AR5 ; Mellati A6 ; Mossahebimohammadi M7 ; Soleimanifar F8 ; Enderami SE9, 10
Authors
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Authors Affiliations
  1. 1. Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
  2. 2. Department of Medical Biotechnology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  3. 3. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Science, Karaj, Iran
  4. 4. Department of Medical Genetics and Molecular Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  5. 5. Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  6. 6. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  7. 7. International Collaborative Center on Growth Factor Research, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China
  8. 8. Department of Medical Biotechnology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
  9. 9. Molecular and Cell biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  10. 10. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Source: Polymers for Advanced Technologies Published:2020


Abstract

Using stem cells to replace the lost beta cells is a hopeful strategy in the treatment of diabetic patients. Furthermore, during stem cell culture and therapy, it is a need to use a substrate to act as a supportive matrix to mimic 3D in vivo microenvironment. Therefore, in this study, human adipose-derived stem cells were used to differentiate into insulin-producing cells (IPCs) on a silk/polyethersulfone (PES) scaffold. After exposing to the differentiation media, 2D and 3D (silk/PES) cultured cells were gradually aggregated and formed spherical shaped clusters. The viability of cells was comparable in both 3D and 2D culture. As the results of gene expression assay in both RNA and protein level showed, the differentiation efficiency was higher in 3D culture. Furthermore, ELISA revealed that the release of C-peptide and insulin was higher in 3D than 2D culture. It seems that silk/PES nanofibrous hybrid scaffold could provide an appropriate matrix to mimic in vivo microenvironment and therefore increases the IPC differentiation potency of stem cells. © 2020 John Wiley & Sons Ltd
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