Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Sex-Dependent Paracrine Effect of Conditioned Media From Adipose Tissue Derived Mesenchymal Stem Cells on Prostate Cancer Cells Publisher Pubmed



Mirzaei A1 ; Mashhadi R1 ; Aghsaeifard Z1 ; Izadi M1 ; Dougaheh SNH1 ; Omid R1 ; Guitynavard F1 ; Nikoofar P2 ; Aghamir SMK1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Urology, Thunder Bay Regional Health Research Institute, Thunder Bay, ON, Canada

Source: Journal of Cellular and Molecular Medicine Published:2025


Abstract

Considering the different behaviour of cells in response to diseases in different conditions and sex hormone-dependent cancers, we addressed the possible effect of the sex of the source of these cells from adipose tissue on prostate cancer cells. In this in vitro study, we evaluated the effects of male and female MSC Conditioned Media (MCM, FCM) on prostate cancer cells. The assessment included Hoechst dye staining, a scratch-wound assay, a colony formation assay, and a flow cytometric analysis of apoptosis and the cell cycle. We also performed real-time PCR to examine various genes, including apoptosis-related genes, epithelial-mesenchymal transition (EMT) genes, angiogenesis-related genes, and cell growth and survival biomarkers. Our results indicated that the IC50 values were 50% and 75% media in MCM and FCM in each of the three prostate cancer cell lines, respectively. An evaluation of gene expression revealed that in all three prostate cancer cell lines, treatment with MCM was more effective than FCM in reducing the expression of N-Cadherin and Vimentin, EGFR and BCL2 genes (p < 0.001). Furthermore, the MCM significantly increased the expression of BAX and E-Cadherin genes (p < 0.001) in the PC3 cell line. MCM proved to be more effective than FCM in reducing the expression of the epithelial-mesenchymal transition pathway, EGFR gene, and Apoptosis Regulator (BCL2) in the PC3 cell line. Due to its potential in regenerative medicine and cell therapy, this approach may serve as an effective treatment option for advanced prostate cancer. © 2025 The Author(s). Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
Related Docs
1. Effect of 17Β-Estradiol on Mediators Involved in Mesenchymal Stromal Cell Trafficking in Cell Therapy of Diabetes, Cytotherapy (2015)
2. The Effect of Mesenchymal Stem Cells-Derived Exosomes on the Prostate, Bladder, and Renal Cancer Cell Lines, Scientific Reports (2022)
3. Immunopathology of Type 1 Diabetes and Immunomodulatory Effects of Stem Cells: A Narrative Review of the Literature, Endocrine# Metabolic and Immune Disorders - Drug Targets (2022)
4. Therapeutic and Immunomodulatory Potentials of Mesenchymal Stromal/Stem Cells and Immune Checkpoints Related Molecules, Biomarker Research (2024)
5. Recent Advances in Mesenchymal Stem Cell Therapy for Multiple Sclerosis: Clinical Applications and Challenges, Frontiers in Cell and Developmental Biology (2025)
6. Mesenchymal Stromal Cells in Bone Marrow Niche of Patients With Multiple Myeloma: A Double-Edged Sword, Cancer Cell International (2025)
7. Cancer-Associated Mesenchymal Stem/Stromal Cells: Role in Progression and Potential Targets for Therapeutic Approaches, Frontiers in Immunology (2023)
8. Signaling Crosstalk Between Mesenchymal Stem Cells and Tumor Cells: Implications for Tumor Suppression or Progression, Cytokine and Growth Factor Reviews (2024)
Experts (# of related papers)
View all Related Experts
Hamidreza Aghayan (1)
Samira Rajaei (1)