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Construction of a Recombinant B-Cell Epitope Vaccine Based on a Der P1-Derived Hypoallergen: A Bioinformatics Approach Publisher Pubmed



Fanuel S1, 2 ; Tabesh S4 ; Mokhtarian K5 ; Saroddiny E1 ; Fazlollahi MR2 ; Pourpak Z2 ; Falak R3 ; Kardar GA1, 2
Authors
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Authors Affiliations
  1. 1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences - International Campus (IC-TUMS), Tehran, Iran
  2. 2. Immunology, Asthma and Allergy Research Institute (IAARI), Tehran University of Medical Science, Tehran, Iran
  3. 3. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Medicinal Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

Source: Immunotherapy Published:2018


Abstract

Aim: House dust mite (HDM) allergens are important elicitors of IgE-mediated allergies. This study was aimed at constructing and characterizing a recombinant fusion protein, DpTTDp, which was based on carrier-bound Der p 1-derived peptides for HDM allergen immunotherapy. Methods: Using the Immune Epitope Database (IEDB), we identified from Der p 1, a 34-mer hypoallergenic peptide. Two copies of the hypoallergen were then fused to a partial fragment of a tetanus toxoid molecule's N-and C terminus and expressed in Escherichia coli. After purification to homogeneity, the protein was evaluated for allergenicity and its ability to induce blocking antibodies upon immunization. Results: Upon immunization of mice, DpTTDp induced high levels of protective IgG-antibodies that blocked allergic patients' IgE reactivity to HDM. In addition, DpTTDp lacked relevant IgE-reactivity, induced low T-cell proliferation and IFN-γ in peripheral blood mononuclear cells of HDM-allergic patients' sera. Conclusion: The protein represents a promising HDM-allergy immunotherapy candidate vaccine. © 2018 Future Medicine Ltd.
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