Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Lighter Than Water Dispersive Liquid-Liquid Microextraction Coupled With High Performance Liquid Chromatography for Determination of Cholecalciferol and Calcifediol From Plasma



Pazhohan A1 ; Mogaddam MRA2, 3 ; Amidi F4 ; Jafarzadeh S1 ; Farajzadeh MA5, 6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Infertility center, Academic Center for Education, Culture and Research East Azarbaijan, Tabriz, Iran
  2. 2. Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
  6. 6. Engineering Faculty, Near East University, North Cyprus, Mersin 10, Nicosia, 99138, Turkey

Source: Analytical and Bioanalytical Chemistry Research Published:2020

Abstract

In this study, a dispersive liquid-liquid microextraction method using an extraction solvent lighter than water has been developed for the extraction and preconcentration of cholecalciferol and calcifediol from plasma samples followed by high performance liquid chromatography determination. Initially, acetonitrile and sodium chloride (NaCl) are added into the plasma as an extraction solvent and a salting-out agent, respectively. After manual shaking, the mixture is centrifuged. In the presence of sodium chloride, a two-phase system is formed. Then, a portion of the upper phase is removed and mixed with n-hexane at μl-level and rapidly injected into distilled water by a syringe. In this process, the analytes are extracted into the fine droplets of n-hexane (as an extraction solvent). Under optimal conditions, enrichment factor was obtained 92 and 94 for calcifediol and cholecalciferol, respectively. The intra-(n = 6) and inter-day (n = 4) precisions were less than or equal to 8.1% at a concentration of 10 ng ml-1 of each analyte. Finally, this method was applied to the analysis of the analytes in human plasma samples. © 2020 Iranian Chemical Society.