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Unique Cd44 Intronic Snp Is Associated With Tumor Grade in Breast Cancer: A Case Control Study and in Silico Analysis Publisher



Esmaeili R1 ; Abdoli N1, 2 ; Yadegari F1 ; Neishaboury M3 ; Farahmand L1 ; Kaviani A3 ; Majidzadeha K1
Authors
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Authors Affiliations
  1. 1. Motamed Cancer Institute, ACECR, Genetics Department, Breast Cancer Research Center, No 146, South Gandhi Ave, Vanak Sq., Tehran, Iran
  2. 2. Azad University of Karaj, Tehran, Iran
  3. 3. Tehran University of Medical Sciences, Department of Surgery, No 136, Molla-Sadra St., Tehran, Iran

Source: Cancer Cell International Published:2018


Abstract

Background: CD44 encoded by a single gene is a cell surface transmembrane glycoprotein. Exon 2 is one of the important exons to bind CD44 protein to hyaluronan. Experimental evidences show that hyaluronan-CD44 interaction intensifies the proliferation, migration, and invasion of breast cancer cells. Therefore, the current study aimed at investigating the association between specific polymorphisms in exon 2 and its flanking region of CD44 with predisposition to breast cancer. Methods: In the current study, 175 Iranian female patients with breast cancer and 175 age-matched healthy controls were recruited in biobank, Breast Cancer Research Center, Tehran, Iran. Single nucleotide polymorphisms of CD44 exon 2 and its flanking were analyzed via polymerase chain reaction and gene sequencing techniques. Association between the observed variation with breast cancer risk and clinico-pathological characteristics were studied. Subsequently, bioinformatics analysis was conducted to predict potential exonic splicing enhancer (ESE) motifs changed as the result of a mutation. Results: A unique polymorphism of the gene encoding CD44 was identified at position 14 nucleotide upstream of exon 2 (A37692→G) by the sequencing method. The A>G polymorphism exhibited a significant association with higher-grades of breast cancer, although no significant relation was found between this polymorphism and breast cancer risk. Finally, computational analysis revealed that the intronic mutation generated a new consensus-binding motif for the splicing factor, SC35, within intron 1. Conclusions: The current study results indicated that A>G polymorphism was associated with breast cancer development; in addition, in silico analysis with ESE finder prediction software showed that the change created a new SC35 binding site. © 2018 The Author(s).
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