Electrospun Polyurethane/Collagen/Chitosan Nanofibers Containing Gold Nanoparticles for Cardiac Tissue Regeneration Publisher



Khanbaba A^{1, 2} ; Mozaffari H^{1, 2} ; Faridimajidi R³ ; Derakhshan MA¹ Show Affiliations
Source: BioNanoScience Published:2025

Abstract

As the regeneration capability of cardiac tissue after myocardial infarction is limited, nanofibrous cardiac patches have been introduced for a complete tissue reconstruction. In the present study, electrospun nanofibrous scaffolds were fabricated out of polyurethane, chitosan, collagen, and saffron extract-synthesized gold nanoparticles. Successful AuNP synthesis was shown by UV–Vis spectroscopy and the nanoparticles illustrated a hydrodynamic size and zeta potential of around 13 nm and − 37 mV, respectively. Morphology and diameter of the obtained random and aligned nanofibers were observed by SEM. Further, ATR-FTIR, XRD, and TGA investigations were performed. In vitro degradation study of the scaffolds was done to illustrate their weight loss during 60 days immersion in PBS and morphological changes by SEM. Hydrophilic nature of the nanofibers containing chitosan, collagen, and AuNPs was demonstrated using water contact angle measurements. Also, proper tensile properties of the nanofibrous scaffolds were confirmed for potential application as cardiac patches. Finally, alamar blue assay was performed in three pre-set times to evaluate the viability of HUVEC cells on the scaffolds. The findings showed the appropriate survival of the cells on the fabricated nanofibrous scaffolds. Therefore, it could be concluded that PU/Cs/AuNPs and PU/Col/Cs/AuNPs nanofibrous scaffolds could be considered for further studies as potential cardiac patches. Graphical Abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.