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Fabrication of Graphene-Silver/Polyurethane Nanofibrous Scaffolds for Cardiac Tissue Engineering Publisher



Nazari H1, 2 ; Azadi S3, 4 ; Hatamie S1 ; Zomorrod MS1 ; Ashtari K5 ; Soleimani M2 ; Hosseinzadeh S6
Authors
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Authors Affiliations
  1. 1. Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Center, Tehran, Iran
  2. 2. Department of Cell Therapy and Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Faculty of Biomedical Engineering, AmirKabir University of Technology, Tehran, Iran
  4. 4. Faculty of biomedical Engineering, University of Technology Sydney, Sydney, NSW, Australia
  5. 5. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Polymers for Advanced Technologies Published:2019


Abstract

The graphene-based nanocomposites are considered as great candidates for enhancing electrical and mechanical properties of nonconductive scaffolds in cardiac tissue engineering. In this study, reduced graphene oxide-silver (rGO-Ag) nanocomposites (1 and 2 wt%) were synthesized and incorporated into polyurethane (PU) nanofibers via electrospinning technique. Next, the human cardiac progenitor cells (hCPCs) were seed on these scaffolds for in vitro studies. The rGO-Ag nanocomposites were studied by X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscope (TEM). After incorporation of rGO-Ag into PU nanofibers, the related characterizations were carried out including scanning electron microscope (SEM), TEM, water contact angle, and mechanical properties. Furthermore, PU and PU/nanocomposites scaffolds were used for in vitro studies, wherein hCPCs showed good cytocompatibility via 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and considerable attachment on the scaffold using SEM studies. Real-time polymerase chain reaction (PCR) and immunostaining studies confirmed the upregulation of cardiac specific genes including GATA-4, T-box 18 (TBX 18), cardiac troponin T (cTnT), and alpha-myosin heavy chain (α-MHC) in the PU/rGO-Ag scaffolds in comparison with neat PU ones. Therefore, these nanofibrous rGO-Ag–reinforced PU scaffolds can be considered as suitable candidates in cardiac tissue engineering. © 2019 John Wiley & Sons, Ltd.
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