The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells

The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells Publisher Pubmed

Tavakol S¹ ; Modarres Mousavi SM^{2, 3} ; Massumi M^{4, 5} ; Amani A¹ ; Rezayat SM^{1, 6, 7} ; Ai J^{8, 9}

Show Affiliations

1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Shefa Neuroscience Research Center, Khatam Al-Anbia Hospital, Tehran, Iran
3. Shiraz University, Shiraz, Iran
4. Induced Pluripotent Stem Cell Biotechnology Team, Department of Nanobiomaterials and Tissue Engineering, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
5. Stem Cells Biology Department, Stem Cell Technology Research Center, Tehran, Iran
6. Department of Toxicology and Pharmacology, School of Pharmacy, Islamic Azad University (IAUPS), Tehran, Iran
7. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
8. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
9. Brain and Spinal Injury Research Center, Imam Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Biomedical Materials Research - Part A Published:2015

Abstract

A very important obstacle in axonal regeneration after spinal cord injury is astroglial scaring. Noggin as bone morphogenic protein inhibitor plays a critical role in decreasing GFAP+ cells and reducing the number of astrocytes in the site of injury. Human endometrial-derived stromal cells (hEnSCs) were isolated and cultured in two different neural inductive mediums consisting of neural progenitor maintenance medium (NPMM)/BDNF or NPMM/BDNF/Noggin in Matrigel 3D cell culture. Neural expression markers were investigated at the mRNA and protein level by real-time PCR and immunocytochemistry, respectively. The results showed that Noggin supplementation was able to increase the expression of Nestin, Tuj-1, and NF, whereas the expressions of GFAP, Bcl2, and Olig2 were decreased. In addition, DAPI staining demonstrated that lighter blue chromatin agreed with our observation of lower level of Bcl2 expression in the Noggin protocol in which over-expression of Bcl2 gene did not induce higher neurogenesis in poor Noggin medium. Our findings clearly demonstrated the neural differentiation potential of hEnSC in Matrigel and also Noggin supplementation was able to inhibit astrocyte formation. © 2014 Wiley Periodicals, Inc.

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Style	Citing Format
MLA	Tavakol S, et al.. "The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells." Journal of Biomedical Materials Research - Part A, vol. 103, no. 1, 2015, pp. 1-7.
APA	Tavakol S, Modarres Mousavi SM, Massumi M, Amani A, Rezayat SM, Ai J (2015). The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells. Journal of Biomedical Materials Research - Part A, 103(1), 1-7.
Chicago	Tavakol S, Modarres Mousavi SM, Massumi M, Amani A, Rezayat SM, Ai J. "The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells." Journal of Biomedical Materials Research - Part A 103, no. 1 (2015): 1-7.
Harvard	Tavakol S et al. (2015) 'The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells', Journal of Biomedical Materials Research - Part A, 103(1), pp. 1-7.
Vancouver	Tavakol S, Modarres Mousavi SM, Massumi M, Amani A, Rezayat SM, Ai J. The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells. Journal of Biomedical Materials Research - Part A. 2015;103(1):1-7.
BibTex	@article{ author = {Tavakol S and Modarres Mousavi SM and Massumi M and Amani A and Rezayat SM and Ai J}, title = {The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells}, journal = {Journal of Biomedical Materials Research - Part A}, volume = {103}, number = {1}, pages = {1-7}, year = {2015} }
RIS	TY - JOUR AU - Tavakol S AU - Modarres Mousavi SM AU - Massumi M AU - Amani A AU - Rezayat SM AU - Ai J TI - The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells JO - Journal of Biomedical Materials Research - Part A VL - 103 IS - 1 SP - 1 EP - 7 PY - 2015 ER -

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