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Oleic Acid Promotes the Expression of Neural Markers in Differentiated Human Endometrial Stem Cells Publisher Pubmed



Kojour MAM1 ; Barough SE2 ; Kouchesfehani HM1 ; Jalali H1 ; Ebrahim MHK1
Authors
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Authors Affiliations
  1. 1. Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
  2. 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advance Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Chemical Neuroanatomy Published:2017


Abstract

Variety of neurodegenerative diseases in humans are caused by loss of cells along with loss of function and disability. Cell replacement therapy is a potential strategy to cure neurodegenerative diseases. Mesenchymal stem cells are pluripotent non-hematopoietic cells that can be isolated from numerous tissues. Human endometrial-derived stem cell (hEnSC) are the abundant and easy available source with no immunological response, for cell replacement therapy. In the nervous system, where fatty acids are found in huge amounts, they participate in its development and maintenance throughout life. Oleic acid is a kind of the saturated fatty acids which plays crucial role in brain development. Oleic acid released by astrocytes is used by neurons for the synthesis of phospholipids and is specifically incorporated into growth cones. Human endometrial-derived stem cells in the third passage were divided into 3 groups including: control, sham (cultured in full differentiation medium without oleic acid) and experimental group (cultured in full differentiation medium with oleic acid) to differentiate over a 18-day period. Data from Real-Time PCR showed that mRNA levels of NF and β-TUBULIN were increased significantly (p < 0.05) in oleic acid treated cells in comparison to control and sham groups. Immunocytochemistry analysis of Chat and NF expression also showed the same results. The present study clearly demonstrates that oleic acid promotes neural differentiation of hEnSC through regulation of gene expression. © 2016 Elsevier B.V.
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