Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Determine Antibiotic Resistance Model and Identify Methicillin-Resistant Staphylococcus Aureus (Mrsa) in Clinical Isolates



Effatpanah H1 ; Mohammadi MJ2, 3 ; Safari N4 ; Mahmodi A5 ; Seidmohammadi A6 ; Bozorgomid A7 ; Effatpanah M8
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Public Health, School of Asadabad Medical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Department of Environmental Health Engineering, School of Public Health and Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  3. 3. Asadabad School of Medical Sciences, Asadabad, Iran
  4. 4. Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran
  5. 5. Clinical Laboratory Science, Hamadan University of Medical Sciences, Hamadan, Iran
  6. 6. Social Determinants of Health Research Center, School of Public Health, Hamadan University of Medical Sciences, Iran
  7. 7. Department of Microbiolgy, Asadabad School of Medical Sciences, Asadabad, Iran
  8. 8. School of Medicine, Ziaeian Hospital, International Campus, Tehran University of Medical Sciences, Tehran, Iran

Source: Fresenius Environmental Bulletin Published:2018

Abstract

Nosocomial infection, especially treatment-resistant forms is a serious problem in the health system and can impose much cost on countries' the health sector. The purpose of this study is to determine antibiotic resistance model and identify mecA gene in clinical isolates of Staphylococcus aureus. In this cross sectional study conducted in September and October 2016, 96 negative and positive coagulase staphylococci isolates were collected from clinical, environmental and personnel samples in wards CCU, dialysis, surgery and internal of Hazrat Ali (AS) Hospital in Asadabad, west of Iran. Antibiotic susceptibility model of the isolates were examined by 5 antibiotic discs and the minimum inhibitory concentration of antibiotic oxacillin was used on Mueller Hinton agar, after extracting DNA PCR test was used for methicillin-resistance gene for Staphylococcus aureus. Among 96 isolates collected, 65 isolates were Staphylococcus coagulase negative and 31 isolates were Staphylococcus aureus of which 8 isolates (25%) were mecA gene productive. The highest resistance rate was related to antibiotic oxacillin (8 isolates) and the lowest resistance rate was related to antibiotic trimethoprim-sulfamethoxazole (zero). In none of the isolates, no strain was reported as MDR. The results indicate that the prevalence of Staphylococcus aureus was significant in nosocomial samples and resistance to methicillin is increasing in the bacteria strains. © by PSP.