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Recent Developments In Recombinant Proteins for Diagnosis of Human Fascioliasis Publisher Pubmed



Mirzadeh A1 ; Jafarihaghighi F2 ; Kazemirad E3 ; Sabzevar SS4 ; Tanipour MH1 ; Ardjmand M2
Authors
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Authors Affiliations
  1. 1. Department of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, 3010, VIC, Australia
  2. 2. Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, 1584743311, Iran
  3. 3. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Acta Parasitologica Published:2021


Abstract

Fascioliasis is an important neglected tropical disease that causes severe injury to the bile ducts and liver. Therefore, a rapid and accurate method for detection of Fasciola hepatica infection plays a vital role in early treatment. Currently, the diagnosis of fascioliasis is mainly conducted via serological tests using the excretory/secretory (E/S) products, which might cross-react with antigens from other helminth parasitic diseases. Hence, the development of serodiagnosis test using recombinant antigens may contribute to differentiate fascioliasis from other helminth infections. In the past 20 years, many attempts have been made to exert different F. hepatica recombinant antigens to obtain a well-established standard assay with high accuracy. In this review, we address recent studies that refer to the development of serodiagnosis tests for diagnosis of human fascioliasis based on the candidate recombinant antigens produced by different approaches. Meanwhile, in the present review, some main factors have been highlighted to improve the accuracy of diagnostic tests such as the effect of refolding methods to recover antigens’ tertiary structure as well as applying a mixture of recombinant antigens with the highest sensitivity and specificity to improve the accuracy of diagnostic tests. © 2020, Witold Stefanski Institute of Parasitology, Polish Academy of Sciences.
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