Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran

Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran Publisher

Mir S¹ ; Dabirzadeh M² ; Rokni MB^{3, 4} ; Aryaeipour M³ ; Khoshsima Shahraki M² ; Azizi H²

Show Affiliations

1. Student Research Committee, Zabol University of Medical Sciences, Zabol, Iran
2. Department of Medical Parasitology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
3. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Public Health Published:2019

Abstract

Background: The detection of Fasciola species in various geographical regions is essential for health policymak-ing. Here, we aimed to identify livestock (cattle and sheep) related Fasciola genotypes by restriction fragment length polymorphism PCR. Methods: Seventy adult Fasciola flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaugh-tered in Zabol abattoir, outh-east Iran (Jan-Jul 2017). Fasciola species were determined based on molecular fea-tures. For molecular detection, Fasciola ITS1 region was amplified and sequenced. A 700 bp fragment was ampli-fied. These were digested with RasI enzyme. F. hepatica specific fragments were 47, 59, 68, 104, and 370, while those related to F. gigantica had 45, 55, 170, 370. Results: The two main species of F. hepatica and F. gigantica are responsible for fasciolosis in sheep and cattle in our region. From 35 Fasciola isolated from cattle, 3 and 32 were F. hepatica and F. giagantica respectively. From 35 Fasciola isolated from sheep, 4 were F. hepatica and 31 were F. gigantica. Conclusion: All Seventy Fasciola samples from two different hosts (cattle and sheep) were identified as either F. hepatica or F. gigantica by PCR-RFLP. Genotypic variability of Fasciola species was high in our region. It is rec-ommended to assess molecular variation of Fasciola isolates in other host livestock. © 2019, Iranian Journal of Public Health. All rights reserved.

Related Docs

View other Related Docs

1. Identification and Differentiation of Fasciola Hepatica and F. Gigantica Using Multiplex Pcr Technique, Annals of Parasitology (2023)

2. Molecular Characterization of Animal Fasciola Spp. Isolates From Kermanshah,Western Iran, Iranian Journal of Public Health (2016)

3. Morphological and Molecular Characterization of Fasciola Isolates From Livestock in Golestan Province, Northern Iran, Veterinary Medicine and Science (2023)

4. Molecular Characterization of Animal Fasciola Spp. Isolates From Lorestan Province, Western Iran, Iranian Journal of Public Health (2022)

5. Identification of Fasciola Species Using Tegumental Spines in Tissue Sections, Iranian Journal of Public Health (2020)

6. Molecular Characterization of Fasciola Spp. From Some Parts of Iran, Iranian Journal of Public Health (2020)

7. Comparative Evaluation of Real-Time Pcr and Elisa for the Detection of Human Fascioliasis, Scientific Reports (2024)

8. Seroprevalence of Human Fasciolosis in Pirabad, Lorestan Province, Western Iran, Iranian Journal of Parasitology (2016)

Experts (# of related papers)

View all Related Experts

Mahdi Mohebali (6)

Eshrat Beigom Kia (3)

Style	Citing Format
MLA	Mir S, et al.. "Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran." Iranian Journal of Public Health, vol. 48, no. 5, 2019, pp. 934-942.
APA	Mir S, Dabirzadeh M, Rokni MB, Aryaeipour M, Khoshsima Shahraki M, Azizi H (2019). Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran. Iranian Journal of Public Health, 48(5), 934-942.
Chicago	Mir S, Dabirzadeh M, Rokni MB, Aryaeipour M, Khoshsima Shahraki M, Azizi H. "Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran." Iranian Journal of Public Health 48, no. 5 (2019): 934-942.
Harvard	Mir S et al. (2019) 'Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran', Iranian Journal of Public Health, 48(5), pp. 934-942.
Vancouver	Mir S, Dabirzadeh M, Rokni MB, Aryaeipour M, Khoshsima Shahraki M, Azizi H. Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran. Iranian Journal of Public Health. 2019;48(5):934-942.
BibTex	@article{ author = {Mir S and Dabirzadeh M and Rokni MB and Aryaeipour M and Khoshsima Shahraki M and Azizi H}, title = {Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran}, journal = {Iranian Journal of Public Health}, volume = {48}, number = {5}, pages = {934-942}, year = {2019} }
RIS	TY - JOUR AU - Mir S AU - Dabirzadeh M AU - Rokni MB AU - Aryaeipour M AU - Khoshsima Shahraki M AU - Azizi H TI - Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran JO - Iranian Journal of Public Health VL - 48 IS - 5 SP - 934 EP - 942 PY - 2019 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract