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Protein Detection of Excretory-Secretory Products and Somatic Extracts From Fasciola Hepatica and F. Gigantica Using Two-Dimensional Electrophoresis Publisher



Rasouli A1 ; Farahnak A1 ; Zali H2 ; Rezaeian M1 ; Golestani A3 ; Molaei Rad MB1
Authors
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Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Proteomics Research Center, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Parasitology Published:2019


Abstract

Background: The aim of this research was to compare excretory-secretory and somatic extract materials of Fasciola hepatica and F. gigantica to detect protein maps of two species. Methods: Twenty infected livers were collected from sheep in industrial slaughter-house in Tehran, 2017-2019. Worms were detached from bile ducts, then recog-nized according to morphologic and morphometric criteria. After three times wash-ing, worms were incubated in RPMI culture media and excretory-secretory prod-ucts were collected. Worms were crushed and homogenized for preparation of so-matic extract. Two Dimensional Electrophoresis gels were accomplished for both excretory-secretory material and somatic extracts. Gels were scanned with densi-tometer and analyzed with Same Spots software and protein spots were identified with Expasy database. Results: For both excretory-secretory products and somatic extract, protein spots were appeared with two-dimensional electrophoresis technique. Quantitative analy-sis showed 40 and 28 protein spots for excretory-secretory of F. hepatica and F. gi-gantica respectively. For somatic extract 19 and 12 protein spots were recognized for F. hepatica and F. gigantica in that order. Conclusion: The rate of expression of some proteins were more in F. hepatica while expression of other proteins was high in F. gigantica. The expression of protease enzyme was higher in F. gigantica than F. hepatica. These data could be considered for biochemi-cal differentiation of Fasciola species and subsequently to design and prepare of antigens for diagnosis/vaccine development. © 2019, Tehran University of Medical Sciences (TUMS). All rights reserved.