Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells

Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells Publisher Pubmed

Khaksarian M^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Mostafavi H^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Soleimani M^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Karimian SM^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Ghahremani MH^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Joghataee MT^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Khorashadizadeh M^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Aligholi H^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Attari F^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10} ; Hassanzadeh G^{1, 2, 3, 4, 5, 6, 7, 8, 9, 10}

Show Affiliations

1. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
2. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
3. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
4. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
5. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
6. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
7. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
8. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
9. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran
10. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 1417743371, Iran

Source: Molecular medicine reports Published:2015

Abstract

Connexin 43 (Cx43) is the main gap junction protein in astrocytes and exerts the same effects on growth inhibition in astrocytoma and glioma as microRNA-146a (miR-146a) in glioma. β2-adrenergic receptor (AR) signaling modulates Cx43 expression in myocytes via components downstream of protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac). However, it remains to be elucidated how expression of Cx43 is modulated in astrocytes. In the present study, 1321N1 astrocytoma cells were treated with β2-AR signaling agents in order to evaluate the expression of Cx43 and miRNAs. RNA and protein were extracted from the cells for use in reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. The results revealed that clenbuterol increased miR-146a level and upregulated Cx43 expression via cAMP/PKA at the mRNA and protein level. Pre-inhibition of adenyl cyclase decreased expression of Cx43 and miR-146a. PKA activation and overexpression of miR-146a in A-1321N1 cells increased the expression of Cx43. β2-AR stimulation and 6Bnz, a PKA activator, suppressed oncomiRs miR-155 and miR-27a, while 8-(4-chlorophenylthio)-2'-O-methyladenosine-3',5'-cyclic monophosphate, an Epac activator, increased their levels. The current findings demonstrated that β2-AR signaling has growth inhibitory effects via modulation of the cAMP/PKA pathway in A-1321N1 cells through increasing the expression level of Cx43 and miR-146a as well as decreasing miR-155 and miR-27a levels. Thus, stimulation of the β2-AR and PKA signaling pathway may be a useful approach for astrocytoma therapy.

Style	Citing Format
MLA	Khaksarian M, et al.. "Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells." Molecular medicine reports, vol. 12, no. 2, 2015, pp. 1941-1950.
APA	Khaksarian M, Mostafavi H, Soleimani M, Karimian SM, Ghahremani MH, Joghataee MT, Khorashadizadeh M, Aligholi H, Attari F, Hassanzadeh G (2015). Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells. Molecular medicine reports, 12(2), 1941-1950.
Chicago	Khaksarian M, Mostafavi H, Soleimani M, Karimian SM, Ghahremani MH, Joghataee MT, Khorashadizadeh M, Aligholi H, Attari F, Hassanzadeh G. "Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells." Molecular medicine reports 12, no. 2 (2015): 1941-1950.
Harvard	Khaksarian M et al. (2015) 'Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells', Molecular medicine reports, 12(2), pp. 1941-1950.
Vancouver	Khaksarian M, Mostafavi H, Soleimani M, Karimian SM, Ghahremani MH, Joghataee MT, et al.. Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells. Molecular medicine reports. 2015;12(2):1941-1950.
BibTex	@article{ author = {Khaksarian M and Mostafavi H and Soleimani M and Karimian SM and Ghahremani MH and Joghataee MT and Khorashadizadeh M and Aligholi H and Attari F and Hassanzadeh G}, title = {Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells}, journal = {Molecular medicine reports}, volume = {12}, number = {2}, pages = {1941-1950}, year = {2015} }
RIS	TY - JOUR AU - Khaksarian M AU - Mostafavi H AU - Soleimani M AU - Karimian SM AU - Ghahremani MH AU - Joghataee MT AU - Khorashadizadeh M AU - Aligholi H AU - Attari F AU - Hassanzadeh G TI - Regulation of Connexin 43 and Microrna Expression Via Β2-Adrenoceptor Signaling in 1321N1 Astrocytoma Cells JO - Molecular medicine reports VL - 12 IS - 2 SP - 1941 EP - 1950 PY - 2015 ER -

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