Mir-146A-5P Suppresses Migration and Downregulates Vimentin and Mmp-9 Expression in Gastric Cancer Cells Publisher



A Baghbanzadeh AMIR ; S Rahmani SHIMA ; S Eslami SAHAND ; M Ahmadpour Youshanlui MAHYA ; N Shafiee NADIA ; A Shafiee ATEFE ; A Khalaji AMIRREZA ; B Baradaran BEHZAD
Source: Discover Oncology Published:2025

Abstract

Gastric cancer (GC) is a significant global health issue, characterized by poor prognosis due to its metastatic nature. This study investigates the role of microRNA-146a-5p (miR-146a-5p) in regulating migration and invasion of KATO III GC cells by targeting vimentin and matrix metalloproteinase-9 (MMP-9), critical mediators of epithelial–mesenchymal transition (EMT). miR-146a-5p expression was analyzed in GC cell lines, and functional assays were performed after transfection with a miR-146a-5p mimic. Results showed that miR-146a-5p expression was significantly reduced in GC cells, particularly in KATO III. Transfection with the mimic reduced cell viability, increased apoptosis, and inhibited migration, as shown by wound-healing assays. Additionally, miR-146a-5p downregulated vimentin and MMP-9 mRNA expression. These findings suggest that miR-146a-5p may influence metastatic behavior in GC cells through regulation of EMT-related genes. However, further studies, including protein-level validation and direct target confirmation, are needed. These results provide a basis for future investigation into the potential therapeutic role of miR-146a-5p in GC. © 2025 Elsevier B.V., All rights reserved.