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The Synthesis and Development of Poly(Ε-Caprolactone) Conjugated Polyoxyethylene Sorbitan Oleate-Based Micelles for Curcumin Drug Release: An in Vitro Study on Breast Cancer Cells Publisher



Shadmani N1 ; Gohari S2 ; Kadkhodamanesh A3 ; Ghaderinia P4, 5 ; Hassani M6 ; Sharifyrad M4
Authors
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Authors Affiliations
  1. 1. Trita Nanomedicine Research & Technology Development Center (TNRTC), Zanjan Health Technology Park, Zanjan, Iran
  2. 2. Student Research Center, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  3. 3. School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Research and Technology Development Center of the Motahar Zist Gostar, Islamic Azad University, Zanjan Branch, Zanjan, 45156-58145, Iran
  5. 5. Department of Microbiology, Islamic Azad University, Zanjan Branch, Zanjan, Iran
  6. 6. Department of Pharmaceutical Biomaterials, Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: RSC Advances Published:2023


Abstract

Background: it is now known that curcumin (Cur) has a broad range of biological properties; however, photosensitivity, as well as low bioavailability and short half-life, have limited its clinical application. To overcome these problems the synthesis of poly(ϵ-caprolactone)-Tween 80 (PCL-T) copolymers was performed. Methods: the copolymers of PCL-T were created using the solvent evaporation/extraction technique. Then Cur was loaded in PCL-T micelles (PCL-T-M) by a self-assembly method. The characterization of copolymer and micelles was assessed by gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy (1HNMR), differential scanning calorimetry (DSC), transmission electron microscopy (TEM), and dynamic light scattering (DLS) methods. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was used to indicate the cytotoxicity of the free Cur, PCL-T-M, and Cur-loaded PCL-T-M. Results: TEM analysis showed monodispersed and spherical shapes with a size of about 90 nm. Cur was released from PCL-T-M at pH 7.4 (45%) and 5.5 (90%) during 6 days. After 24 and 48 h, the IC50 of the free Cur, PCL-T-M, and Cur-loaded PCL-T-M on MCF-7 cells were 80.86 and 54.45 μg mL−1, 278.30 and 236.19 μg mL−1, 45.47 and 19.05 μg mL−1, respectively. Conclusion: this study showed that, in the same concentration, the effectiveness of the Cur-loaded PCL-T-M is more than the free Cur, and the nano-system has been able to overcome delivery obstacles of Cur drug. Thus, PCL-T-M can be a candidate as a drug carrier for the delivery of Cur and future therapeutic investigations on breast cancer. © 2023 The Royal Society of Chemistry.