Investigation of Antimicrobial Susceptibility Patterns and Frequency of Bla Oxa Genes in Carbapenem Resistant Acinetobacter Baumannii Strains



Moghadasi M¹ ; Kalantarneyestanaki D¹ ; Rahdar HA² ; Jasemi S² ; Karamizarandi M² ; Feizabadi MM² Show Affiliations
Source: Scientific Journal of Kurdistan University of Medical Sciences Published:2018

Abstract

Bachground and Aim: Acinetobacter baumannii is one of the important caues of nosocomial infection. Resistance to antibiotics specially carbapenem antibiotics is on the increase which can be an alarming sign for healthcare managers and personnel. The aim of this study was to investigate phentypic and genotypic characteristics of carbapenem resistant A. baumannii in the samples obtained from the patients in Imam Khomeini Hospital, Tehran city. Material and Methods: 100 strains were identified as A. baumannii by biochemical tests and molecular methods. Multiplex PCR method was used for identification of the genes of bla OXA family. Drug susceptibility pattern was determined by disk diffusion method. Result: Among 100 isolates, 95(95%) were resistant to carbapenem antibiotics(imipenem and meropenem). PCR method was performed to identify bla OXA family genes. 9 isolates had bla OXA -51 gene while 38 had both bla OXA -51 and bla OXA -23 genes. 20 isolates were positive for bla OXA -51 and bla OXA -24 genes, 6 isolates had bla OXA -51 and bla OXA -58, and 8 isolates had bla OXA -51, bla OXA -24 and bla OXA -23 genes. 7 isolates were positive for bla OXA -51, bla OXA -23 and bla OXA -58. 4 isolates had bla OXA -51and bla OXA -24 and bla OXA -58 genes. We detected bla OXA -51and bla OXA -24 and bla OXA -23 and bla OXA -58 genes in three isolates. Conclusion: According to our results carbapenem resistant Acinetobacter baumannii strains spread increasingly among hospital units. We found that carbapenem resistant Acinetobacter strains with a high resistance rate are common in the hospitals in Iran. © 2018 the Author (s).