Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies

Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies Publisher

Bastani K¹ ; Sakhaee K¹ ; Ahmadpoursamani P¹ ; Khatibi A¹ ; Zahedi P¹ ; Akrami M² ; Jafari SH¹

Show Affiliations

1. Nano-Biopolymers Research Laboratory, School of Chemical Engineering, College of Engineering, University of Tehran, P. Box: 11155-4563, Tehran, Iran
2. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Industrial and Engineering Chemistry Published:2025

Abstract

Doxorubicin (DOX) nanocarriers based on star-shaped terpolymer poly(ε-caprolactone)-b-poly[2-(dimethylamino) ethyl methacrylate]-b-poly (N-isopropyl acrylamide) [PCL-b-PDMAEMA-b-PNIPAAm] (NPs) were synthesized with three degrees of polymerization (DP = 50, 100, and 200) for each block and three different block ratios of PNIPAAm/PDMAEMA (0.25, 1, and 4). Their structures were characterized using FTIR, 1H NMR, GPC, turbidometry, and FE-SEM tests. Moreover, the drug loading capacity and encapsulation efficiency were obtained experimentally at 14.91 % and 75 % for the optimal sample (S5), while the simulation ones accounted for 12.95 % and 69.91 % through novel modeling. The in vitro drug release study demonstrated the thermo- and pH-responsive behaviors of NPs (at temperatures of 308 and 318 K, pHs of 5.5 and 7.4), validating the simulation results. The efficacy of DOX-loaded S5 for human myeloid leukemia treatment (U937 cell line) was investigated through hemolysis, MTT, and flow cytometry. Based on the results, the S5 delivered more efficient therapeutic performance than free DOX against U937 cells. Finally, an innovative in vitro molecular dynamics simulation study assessed the interactions between the human serum albumin, fibrinogen proteins, and S5. Accordingly, the cohesive energy densities for human serum albumin and fibrinogen were measured at 4.42 and 42.52 Cal/cm3, respectively, indicating further fibrinogen adhesion with S5. © 2024 The Korean Society of Industrial and Engineering Chemistry

Style	Citing Format
MLA	Bastani K, et al.. "Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies." Journal of Industrial and Engineering Chemistry, vol. 148, no. , 2025, pp. 456-472.
APA	Bastani K, Sakhaee K, Ahmadpoursamani P, Khatibi A, Zahedi P, Akrami M, Jafari SH (2025). Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies. Journal of Industrial and Engineering Chemistry, 148(), 456-472.
Chicago	Bastani K, Sakhaee K, Ahmadpoursamani P, Khatibi A, Zahedi P, Akrami M, Jafari SH. "Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies." Journal of Industrial and Engineering Chemistry 148, no. (2025): 456-472.
Harvard	Bastani K et al. (2025) 'Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies', Journal of Industrial and Engineering Chemistry, 148(), pp. 456-472.
Vancouver	Bastani K, Sakhaee K, Ahmadpoursamani P, Khatibi A, Zahedi P, Akrami M, et al.. Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies. Journal of Industrial and Engineering Chemistry. 2025;148():456-472.
BibTex	@article{ author = {Bastani K and Sakhaee K and Ahmadpoursamani P and Khatibi A and Zahedi P and Akrami M and Jafari SH}, title = {Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies}, journal = {Journal of Industrial and Engineering Chemistry}, volume = {148}, number = {}, pages = {456-472}, year = {2025} }
RIS	TY - JOUR AU - Bastani K AU - Sakhaee K AU - Ahmadpoursamani P AU - Khatibi A AU - Zahedi P AU - Akrami M AU - Jafari SH TI - Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies JO - Journal of Industrial and Engineering Chemistry VL - 148 IS - SP - 456 EP - 472 PY - 2025 ER -

Style

Citing Format

MLA

Bastani K, et al.. "Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies." Journal of Industrial and Engineering Chemistry, vol. 148, no. , 2025, pp. 456-472.

APA

Bastani K, Sakhaee K, Ahmadpoursamani P, Khatibi A, Zahedi P, Akrami M, Jafari SH (2025). Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies. Journal of Industrial and Engineering Chemistry, 148(), 456-472.

Chicago

Bastani K, Sakhaee K, Ahmadpoursamani P, Khatibi A, Zahedi P, Akrami M, Jafari SH. "Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies." Journal of Industrial and Engineering Chemistry 148, no. (2025): 456-472.

Harvard

Bastani K et al. (2025) 'Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies', Journal of Industrial and Engineering Chemistry, 148(), pp. 456-472.

Vancouver

Bastani K, Sakhaee K, Ahmadpoursamani P, Khatibi A, Zahedi P, Akrami M, et al.. Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies. Journal of Industrial and Engineering Chemistry. 2025;148():456-472.

BibTex

@article{ author = {Bastani K and Sakhaee K and Ahmadpoursamani P and Khatibi A and Zahedi P and Akrami M and Jafari SH}, title = {Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies}, journal = {Journal of Industrial and Engineering Chemistry}, volume = {148}, number = {}, pages = {456-472}, year = {2025} }

RIS

TY - JOUR
AU - Bastani K
AU - Sakhaee K
AU - Ahmadpoursamani P
AU - Khatibi A
AU - Zahedi P
AU - Akrami M
AU - Jafari SH
TI - Star-Shaped Poly (Ε-Caprolactone)-B-Poly [2-(Dimethylamino) Ethyl Methacrylate]-B-Poly (N-Isopropyl Acrylamide) As Ph- and Thermo-Responsive Doxorubicin Nanocarriers for Leukemia Therapy: Experimental and Simulation Studies
JO - Journal of Industrial and Engineering Chemistry
VL - 148
IS -
SP - 456
EP - 472
PY - 2025
ER -

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