Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes

Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes Publisher Pubmed

Nazari B¹ ; Kazemi M² ; Kamyab A³ ; Nazari B¹ ; Ebrahimibarough S² ; Hadjighassem M⁵ ; Norouzijavidan A⁵ ; Ai A⁶ ; Ahmadi A⁷ ; Ai J^{2, 5}

Show Affiliations

1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran
4. Section of Rheumatology, Boston University School of Medicine, Boston, MA, United States
5. Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran
6. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
7. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Biomedical Materials Research - Part B Applied Biomaterials Published:2020

Abstract

The importance of tissue engineering has been established as a promising approach in treating neurodegenerative diseases. The purpose of the current study is to determine the effect of fibrin hydrogel on the differentiation of iPSC into oligodendrocyte. For this purpose, iPSCs transduced by miR-338 expressing lentiviruses. They were treated with basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and platelet-derived growth factor (PDGF)-AA. The process was traced by a 6-day treatment in a mitogen-free medium. At the end of the process, multipolar preoligodendrocytes appeared. In comparison to tissue culture plate (TCP), MTT assay demonstrated a significant increase in the viability of cells cultured in fibrin hydrogel. SEM analysis showed cells with elongated morphology and intertwined intercellular interactions. An immunofluorescent assay confirmed the expression of oligodendrocyte markers Olig2 and O4. In comparison to TCP, real-time PCR data indicated a significant increase in the expression of some markers such as Olig2, MBP, Sox10, and PDGFRα on cells encapsulated in fibrin hydrogel. Overall, the results suggest that fibrin hydrogel improves viability of cells and promotes the differentiation of iPSCs into preoligodendrocytes. Hence, it can be used as an appropriate option in the tissue engineering in order to treat neurodegenerative diseases. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:192–200, 2020. © 2019 Wiley Periodicals, Inc.

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Somayeh Ebrahimi Barough (5)

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Style	Citing Format
MLA	Nazari B, et al.. "Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes." Journal of Biomedical Materials Research - Part B Applied Biomaterials, vol. 108, no. 1, 2020, pp. 192-200.
APA	Nazari B, Kazemi M, Kamyab A, Nazari B, Ebrahimibarough S, Hadjighassem M, Norouzijavidan A, Ai A, Ahmadi A, Ai J (2020). Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 108(1), 192-200.
Chicago	Nazari B, Kazemi M, Kamyab A, Nazari B, Ebrahimibarough S, Hadjighassem M, Norouzijavidan A, Ai A, Ahmadi A, Ai J. "Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes." Journal of Biomedical Materials Research - Part B Applied Biomaterials 108, no. 1 (2020): 192-200.
Harvard	Nazari B et al. (2020) 'Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes', Journal of Biomedical Materials Research - Part B Applied Biomaterials, 108(1), pp. 192-200.
Vancouver	Nazari B, Kazemi M, Kamyab A, Nazari B, Ebrahimibarough S, Hadjighassem M, et al.. Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes. Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2020;108(1):192-200.
BibTex	@article{ author = {Nazari B and Kazemi M and Kamyab A and Nazari B and Ebrahimibarough S and Hadjighassem M and Norouzijavidan A and Ai A and Ahmadi A and Ai J}, title = {Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes}, journal = {Journal of Biomedical Materials Research - Part B Applied Biomaterials}, volume = {108}, number = {1}, pages = {192-200}, year = {2020} }
RIS	TY - JOUR AU - Nazari B AU - Kazemi M AU - Kamyab A AU - Nazari B AU - Ebrahimibarough S AU - Hadjighassem M AU - Norouzijavidan A AU - Ai A AU - Ahmadi A AU - Ai J TI - Fibrin Hydrogel As a Scaffold for Differentiation of Induced Pluripotent Stem Cells Into Oligodendrocytes JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials VL - 108 IS - 1 SP - 192 EP - 200 PY - 2020 ER -

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