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Microrna-2861 and Nanofibrous Scaffold Synergistically Promote Human Induced Pluripotent Stem Cells Osteogenic Differentiation Publisher



Abazari MF1 ; Zare Karizi S2 ; Kohandani M3 ; Nasiri N4 ; Nejati F4 ; Saburi E5 ; Nikpoor AR6 ; Enderami SE7 ; Soleimanifar F8 ; Mansouri V9
Authors
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Authors Affiliations
  1. 1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Biology, Varamin Pishva Branch, Islamic Azad University, Pishva, Varamin, Iran
  3. 3. Department of Biology, Faculty of Biological Sciences, Islamic Azad University, East Tehran Branch, Tehran, Iran
  4. 4. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
  5. 5. Medical Genetics and Molecular Medicine Department, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  6. 6. Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  7. 7. Diabetes Research Center, Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Mazandaran university of Medical Sciences, Sari, Iran
  8. 8. Department of Medical biotechnology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
  9. 9. Proteomics Research Center, Department of Anatomy, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Polymers for Advanced Technologies Published:2020


Abstract

Tissue engineering using new strategies has become a growing and promising method for treating large tissue lesions in the body. On the other hand, microRNAs (miRNAs), which are small non-coding regulatory RNAs, are a new class of genetic materials that can have effective pharmacological roles. The combination of these two themes has created promising prospects for the treatment of diseases. Herein, human induced pluripotent stem cells (iPSCs) were transduced with miRNA-2861 and then the osteogenic differentiation potential of transduced iPSCs and non-transduced iPSCs was investigated while cultured on the electrospun poly lactic-co-glycolic acid (PLGA) nanofibrous scaffold and culture plate. MiR-2861-transduced iPSCs showed a significantly higher viability, mineralization, alkaline phosphatase (ALP) activity, calcium content, and bone-related gene expression in comparison with those iPSCs that non-transduced. The results also indicated that this increase is improved when miR-2861 transduced iPSCs are cultured on the PLGA nanofibrous scaffold synergistically. This synergy was also confirmed by the results obtained from of Western blot analysis. It can be concluded that, miR-2861, by negative regulation of those proteins that decrease/inhibit osteogenic differentiation and PLGA nanofibrous scaffold by preparation of a suitable artificial extracellular matrix, have a great positive impact in improving iPSCs osteogenic differentiation potential and this blend can be proposed to use in bone tissue engineering application. © 2020 John Wiley & Sons Ltd
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