The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process

The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process Publisher Pubmed

Farhadihosseinabadi B¹ ; Gholipourmalekabadi M^{2, 3} ; Salimi M⁴ ; Abdollahifar MA⁴ ; Bagheri M⁵ ; Samadikuchaksaraei A^{2, 3} ; Ghanbarian H^{1, 6} ; Mozafari M³ ; Kazemi B⁶ ; Niknejad H⁷

Show Affiliations

1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Cellular and Molecular Research Centre, Iran Universityof Medical Sciences, Tehran, Iran
3. Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
4. Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti. University of Medical Sciences, Tehran, Iran
5. Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. Cellular and Molecular Biology Research center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7. Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Scientific Reports Published:2020

Abstract

Lacto-n-neotatraose (LNnT) oligosaccharide shows properties such as anti-inflammatory, type 2 immune response induction, induced angiogenesis, and anti-bacterial effects. Here, we hypothesized that the application of LnNT in the skin full-thickness wound can accelerate the healing process through its anti-inflammatory effect as well as induction of type 2 immune responses. In this study, we evaluated the cell viability of fibroblasts in the presence of LNnT. The full-thickness wound model was created by punch biopsy. The mice were treated intradermaly with LNnT at the concentrations of 100 and 200 µg or PBS as a control group. The wounds samples were compared based on the macroscopic and histological evaluations. The amount of collagen deposition and expression of genes involved in type 2 immunity were measured by the hydroxyproline assay and real time PCR method, respectively. Our results showed that LNnT had no negative effect on the cell viability of fibroblasts. LNnT increased the wound closure rate on day 7 post-wounding. H&E stain analysis revealed that mice treated with 200 µg LNnT exhibited better healing score, follicle formation, and lower epidermal thickness index. The mice treated with LNnT exhibited a lower collagen deposition on day 21 and higher collagen content on days 7 and 14 post-treatment. The LNnT groups also exhibited a lower number of neutrophils and a higher number of basal cells and fibroblasts. The expression rate of IL-10, IL-4, and IL-13 was higher in the LNnT groups. These results showed the high potential of LNnT for use in treatment of full-thickness wounds. © 2020, The Author(s).

2. How Preparation and Preservation Procedures Affect the Properties of Amniotic Membrane? How Safe Are the Procedures?, Burns (2020)

Style	Citing Format
MLA	Farhadihosseinabadi B, et al.. "The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process." Scientific Reports, vol. 10, no. 1, 2020, pp. -.
APA	Farhadihosseinabadi B, Gholipourmalekabadi M, Salimi M, Abdollahifar MA, Bagheri M, Samadikuchaksaraei A, Ghanbarian H, Mozafari M, Kazemi B, Niknejad H (2020). The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process. Scientific Reports, 10(1), -.
Chicago	Farhadihosseinabadi B, Gholipourmalekabadi M, Salimi M, Abdollahifar MA, Bagheri M, Samadikuchaksaraei A, Ghanbarian H, Mozafari M, Kazemi B, Niknejad H. "The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process." Scientific Reports 10, no. 1 (2020): -.
Harvard	Farhadihosseinabadi B et al. (2020) 'The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process', Scientific Reports, 10(1), pp. -.
Vancouver	Farhadihosseinabadi B, Gholipourmalekabadi M, Salimi M, Abdollahifar MA, Bagheri M, Samadikuchaksaraei A, et al.. The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process. Scientific Reports. 2020;10(1):-.
BibTex	@article{ author = {Farhadihosseinabadi B and Gholipourmalekabadi M and Salimi M and Abdollahifar MA and Bagheri M and Samadikuchaksaraei A and Ghanbarian H and Mozafari M and Kazemi B and Niknejad H}, title = {The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process}, journal = {Scientific Reports}, volume = {10}, number = {1}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Farhadihosseinabadi B AU - Gholipourmalekabadi M AU - Salimi M AU - Abdollahifar MA AU - Bagheri M AU - Samadikuchaksaraei A AU - Ghanbarian H AU - Mozafari M AU - Kazemi B AU - Niknejad H TI - The in Vivo Effect of Lacto-N-Neotetraose (Lnnt) on the Expression of Type 2 Immune Response Involved Genes in the Wound Healing Process JO - Scientific Reports VL - 10 IS - 1 SP - EP - PY - 2020 ER -

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