Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! By
Burn Wound Healing Effect of Neonatal Fibroblast Cells Seeded Into Chitosan Hydrogel in Diabetic Rats



S Zare SONA ; A Ataei ALI ; R Ahmadi RAHIM
Authors

Source: Journal of Krishna Institute of Medical Sciences University Published:2024

Abstract

Background: Diabetic chronic wounds are the most critical clinical issues. Although a wide range of wound dressings have been designed, few of them act sufficiently in repairing and protecting wounds. Aim and Objectives: This study aimed to investigate the burn wound healing effect of neonatal fibroblasts seeded into chitosan hydrogel in diabetic rats. Material and Methods: Two burn wounds were created in male Wistar diabetic rats. The rats were divided into groups treated with fibroblast cells, chitosan hydrogel, fibroblast cell cultured chitosan hydrogel (chitosan + fibroblasts group), and control untreated group. Macroscopic and microscopic examination, wound area measurement, measurement of skin density and thickness, and also skin elasticity were applied to evaluate wound healing. The data were analyzed using ANOVA. Results: Treatment of diabetic burn wound with chitosan + fibroblasts accelerated healing process by promoting re-epithelialization and reducing inflammatory responses in skin tissue. Skin elasticity, thickness and density were higher in chitosan + fibroblasts group compared with control group 3 weeks after treatment. Conclusion: The results of the present study show that dressing the diabetic burn wound with neonatal fibroblasts seeded into chitosan scaffold plays a fundamental role in wound healing process. © 2024 Elsevier B.V., All rights reserved.
Related Docs
View other Related Docs
1. Efficacy and Safety of Chitosan-Based Bio-Compatible Dressing Versus Nanosilver (Acticoattm) Dressing in Treatment of Recalcitrant Diabetic Wounds: A Randomized Clinical Trial, Dermatologic Therapy (2022)
2. Evaluation of Gene Expression Levels in Diabetic Rat Skin Wound Healing Treated With Chitosan/Curcumin Nanoparticles-Loaded Sodium Alginate/Chitosan Hydrogels, International Journal of Biological Macromolecules (2025)
3. In Vitro and in Vivo Investigation of a Novel Amniotic-Based Chitosan Dressing for Wound Healing, Wound Repair and Regeneration (2018)
Experts (# of related papers)
View all Related Experts
Rassoul Dinarvand (3)
Shima Younespour (1)
Other Related Docs
4. Polyhexamethylene Biguanidine Coated Silver Nanoparticles Embedded Into Chitosan Thiourea/Pva Nanofibers As Wound Healing Mats: In Vitro and in Vivo Studies, Carbohydrate Polymers (2025)
5. Overview of Silk Fibroin Use in Wound Dressings, Trends in Biotechnology (2018)
6. Accelerated Wound Healing in a Diabetic Rat Model Using Decellularized Dermal Matrix and Human Umbilical Cord Perivascular Cells, Acta Biomaterialia (2016)
7. Wharton's Jelly Stem Cells Delivered Via a Curcumin-Loaded Nanofibrous Wound Dressings Improved Diabetic Wound Healing Via Upregulating Vegf and Igf Genes: An in Vitro and in Vivo Study, Regenerative Therapy (2024)
8. Accelerated Healing of Full-Thickness Skin Wounds by Multifunctional Exosome-Loaded Scaffolds of Alginate Hydrogel/Pcl Nanofibers With Hemostatic Efficacy, International Journal of Biological Macromolecules (2025)
9. Fabrication and Evaluation of a Soy Protein Isolate/Collagen/Sodium Alginate Multifunctional Bilayered Wound Dressing: Release of Cinnamaldehyde, Artemisia Absinthium, and Oxygen, ACS Applied Bio Materials (2024)
10. Layered Dermal Reconstitution Through Epigallocatechin 3-Gallate Loaded Chitosan Nanoparticle Within Enzymatically Crosslinked Polyvinyl Alcohol/Collagen Fibrous Mat, Journal of Biomaterials Applications (2022)
11. Levofloxacin Nanoemulsion Gel Has a Powerful Healing Effect on Infected Wound in Streptozotocin-Induced Diabetic Rats, Drug Delivery and Translational Research (2021)
12. Development of Pva/Chitosan-G-Poly (N-Vinyl Imidazole)/Tio2/Curcumin Nanofibers As High-Performance Wound Dressing, Carbohydrate Polymers (2022)
13. Assessment of the Efficacy of an Ll-37-Encapsulated Keratin Hydrogel for the Treatment of Full-Thickness Wounds, ACS Applied Bio Materials (2023)
14. The Effects of Fibrobalastic Stem Cells With Collagen Hydrogel on Burn Wound Healing in Animal Model, Journal of Biological Studies (2023)
15. Antibacterial Performance and in Vivo Diabetic Wound Healing of Curcumin Loaded Gum Tragacanth/Poly(Ε-Caprolactone) Electrospun Nanofibers, Materials Science and Engineering C (2016)
16. Can Regenerative Medicine and Nanotechnology Combine to Heal Wounds? the Search for the Ideal Wound Dressing, Nanomedicine (2017)
17. Skin Wound Healing Following the Spray of Human Abdominal Adipose Tissue-Derived Mesenchymal Stem Cells in Diabetic Male Rat, Koomesh (2022)
18. Cell-Based Wound Dressing: Bilayered Pcl/Gelatin Nanofibers-Alginate/Collagen Hydrogel Scaffold Loaded With Mesenchymal Stem Cells, International Journal of Biological Macromolecules (2023)
19. How Biomimetic Nanofibers Advance the Realm of Cutaneous Wound Management: The State-Of-The-Art and Future Prospects, Progress in Materials Science (2024)
20. Kaolin-Loaded Chitosan/Polyvinyl Alcohol Electrospun Scaffold As a Wound Dressing Material: In Vitro and in Vivo Studies, Journal of Wound Care (2020)