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Healing Potential of Fibroblast Cells Cultured on a Pla/Cs Nanofibrous Scaffold in Skin Regeneration in Wistar Rat



Hoveizi E1 ; Mohammadi T1 ; Ebrahimibarough S2 ; Tavakol S3, 4
Authors
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Authors Affiliations
  1. 1. Dept. of biology, Shahid Chamran University, Ahvaz, Iran
  2. 2. Dept. of Tissue Engineering and Applied Cell Sciences, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Drug Nanocarriers Research Core, Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Advanced Medical Science and Technologies Association, Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Koomesh Published:2016

Abstract

Introduction: Chronic wound treatment has become a major health issue in developed countries, because of their increasing elderly populations. Although chronic wounds are common problem in all population, treatment for these disabling conditions remains limited and largely ineffective. In this study, we examined the benefits of transplantation of fibroblast cells, cultured on PLA/CS (Poly lactic acid/ Chitosan) scaffold, in wound healing. Materials and Methods: In this study, fibroblast cells were cultured on nanofibrous PLA/CS scaffold then transplanted in rat. The PLA/CS scaffold was prepared by electrospinning method. Also histological staining methods were used to evaluate cell density and amount of collagen. Results: The macroscopic observations and histological staining showed that the wound healed much quicker in PLA/CS scaffold in compare to control group. In vivo assessment showed that treatment with fibroblast cell loaded scaffolds significantly promoted cell density and amount of collagen in rat compared to control group. Conclusion: These results indicated that the capacity of nanofibrous PLA/CS scaffold cultured with fibroblast cells in vivo wound healing. © 2016, Semnan University of Medical Sciences. All rights reserved.
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