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Effects of Guluronic Acid (G2013) on Gene Expression of Tlr2, Tlr4, Myd88, Tnf-Α and Cd52 in Multiple Sclerosis Under in Vitro Conditions Publisher Pubmed



Noorbakhsh SM1 ; Razavi A1 ; Moghadam NB2 ; Saadat P3 ; Hoseini M1 ; Aghazadeh Z1 ; Mobini M1 ; Oraei M1 ; Mirshafiey A1, 4
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Neurology, Shahid Beheshti Universiry of Medical Science, Tehran, Iran
  3. 3. Mobility Impairment Research Center, Health Research Institue, Babol University of Medical Sciences, Babol, Iran
  4. 4. Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Immunopharmacology and Immunotoxicology Published:2019


Abstract

Context: Multiple sclerosis (MS) is an autoimmune and chronic inflammatory disease of CNS. The α-L-guluronic acid (G2013) as novel NSAID with immunomodulatory effects has shown its positive effects in various investigations. Objective: Present research aimed to study the potency of G2013 on gene expression of TLR2, TLR4, MyD88, TNF-α and CD52 in PBMCs of MS patients under in vitro conditions. Materials and methods: 24 blood samples from MS patients and healthy controls were considered for RT-PCR and flow cytometry techniques under two different doses of G2013. Results: Our research indicated that this drug could significantly decrease the gene expression of TLR2, TLR4 and TNF-α compared to untreated group. Conclusion: Data demonstrated that the guluronic acid is able to modify the expression levels of TLR2, TLR4 and TNF-α genes to less than the pathogenic boarder line level, which it might be recommended for reducing the pathological process in multiple sclerosis. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
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