Fluorescence Turn-On Detection of Mirna-155 Based on Hybrid Ce-Mof/ Ptnps /Graphene Oxide Serving As Fluorescence Quencher Publisher



Nemati F¹ ; Hosseini M^{1, 2} Show Affiliations
Source: Journal of Photochemistry and Photobiology A: Chemistry Published:2022

Abstract

Rapid, cost-effective, and specific detection of miRNAs is valuable for clinical diagnosis, pathogenesis, and various medical therapies. In this work, a simple and enzyme-free fluorescence probe was constructed for sensitive detection of miRNA-155 by using Ce-MOF @Pt NP@GO as a novel quencher. Our method is based on monitoring the fluorescence of cyanine (Cy5) in Cy5-tagged DNA probe in the presence of its specific target miRNA. Quenched dye labeled DNA probes are released from Ce-MOF@Pt NP@GO after the miRNA target hybridization. This is accompanied by the significant enhancement of fluorescence emission at 670 nm. Accuracy, sensitivity of the method can be affected by hybridization between the probe and target. This developed method offers a relatively rapid, sensitive, and inexpensive platform without enzyme or separation procedures toward the detection of miRNA155. Under optimal conditions, a detection limit of 5 pM and a linear detection range of 0.01–2.5 nM were obtained for miRNA-155 determination. Meanwhile, this approach showed acceptable accuracy and precision for detecting miRNA in seurm samples. © 2022 Elsevier B.V.