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A Label-Free Luminescent Light Switching System for Mirna Detection Based on Two Color Quantum Dots Publisher



Borghei YS1 ; Hosseini M1 ; Ganjali MR2, 3
Authors
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Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran
  2. 2. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
  3. 3. Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Photochemistry and Photobiology A: Chemistry Published:2020


Abstract

In this study, we report a novel switchable fluorescent nanobiosensor for detection of miRNA. The sensor consists of green-emitting and orange-emitting CdTe QDs exhibiting emission spectra at 525 and 599 nm respectively, under excitation wavelength of 360 nm. Following the addition of miRNA targets to the DNA probes and heteroduplex formation of the hybrid, Green fluorescence CdTe QDs were aggregated and quenched by the DNA/miRNA hybrid via strong interactions at their metal centers. Then, with the addition of orange emitting QDs causes the fluorescence energy transfer to occur and was introduced as a ratiometric approach for miR-155 detection. The relative fluorescence intensity ratio was directly proportional to the concentration of miR-155 from 20 to 100 pM and the detection limit was determined at 14.0 pM. The novel nanobiosensor is “light-on”, rapid, and convenient while it does not require modification or separation procedures and can be applied for the detection of miRNAs in cell lysis with satisfactory results. © 2019 Elsevier B.V.
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