Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Comparative Mitochondrial Proteomics of Leishmania Tropica Clinical Isolates Resistant and Sensitive to Meglumine Antimoniate Publisher Pubmed



Tasbihi M1 ; Shekari F2, 3 ; Hajjaran H4 ; Khanmohammadi M5 ; Hadighi R1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  3. 3. Department of Biology, University of Science and Culture, Tehran, Iran
  4. 4. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Laboratory Science, Marand Branch, Islamic Azad University, Marand, Iran

Source: Parasitology Research Published:2020


Abstract

Antimony is an important drug for the treatment of Leishmania parasite infections. In several countries, the emergence of drug-resistant Leishmania species has reduced the effectiveness of this drug. The mechanism of clinical drug resistance is unclear. The aim of this work was to identify mitochondrial proteome alterations associated with resistance against antimonial. A combination of cell fractionation, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and Label-Free Quantification was used to characterize the mitochondrial protein composition of Leishmania tropica field isolates resistant and sensitive to meglumine antimoniate. LC-MS/MS analysis resulted in the identification of about 1200 proteins of the Leishmania tropica mitochondrial proteome. Various criteria were used to allocate about 40% proteins to mitochondrial proteome. Comparative quantitative proteomic analysis of the sensitive and the resistant strains showed proteins with differential abundance in resistance species are involved in TCA and aerobic respiration enzymes, stress proteins, lipid metabolism enzymes, and translation. These results showed that the mechanism of antimony resistance in Leishmania spp. field isolate may be associated with alteration in enzymes involved in mitochondrial pathways. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
Related Docs
View other Related Docs
1. Mitochondrial Proteome Profiling of Leishmania Tropica, Microbial Pathogenesis (2019)
2. Gene Expression Analysis of Antimony Resistance in Leishmania Tropica Using Quantitative Real-Time Pcr Focused on Genes Involved in Trypanothione Metabolism and Drug Transport, Archives of Dermatological Research (2019)
3. Expression Analysis of Activated Protein Kinase C Gene (Lack1) in Antimony Sensitive and Resistant Leishmania Tropica Clinical Isolates Using Real-Time Rt-Pcr, International Journal of Dermatology (2016)
4. The Role of Atp-Binding Cassette Transporter Genes Expression in Treatment Failure Cutaneous Leishmaniasis, AMB Express (2022)
5. Treatment Failure in Cutaneous Leishmaniasis Patients Referred to the School of Public Health, Tehran University of Medical Sciences During 2008-2017, Journal of Arthropod-Borne Diseases (2020)
6. Overexpression of Iron Super Oxide Dismutases A/B Genes Are Associated With Antimony Resistance of Leishmania Tropica Clinical Isolates, Iranian Journal of Parasitology (2022)
7. Proteomic-Based Studies on Leishmania, Journal of Mazandaran University of Medical Sciences (2018)
8. Comparison of Proteome Profiling of Two Sensitive and Resistant Field Iranian Isolates of Leishmania Major to Glucantime® by 2-Dimensional Electrophoresis, Iranian Journal of Parasitology (2015)
Experts (# of related papers)
View all Related Experts
Mahdi Mohebali (13)
Homa Hajjaran (12)
Other Related Docs
9. The Geographical Distribution of Human Cutaneous and Visceral Leishmania Species Identified by Molecular Methods in Iran: A Systematic Review With Meta-Analysis, Frontiers in Public Health (2021)
10. Insights Into the Trypanothione System in Antimony-Resistant and Sensitive Leishmania Tropica Clinical Isolates, Acta Tropica (2024)
11. Comparative Proteomic Profiling of Leishmania Tropica: Investigation of a Case Infected With Simultaneous Cutaneous and Viscerotropic Leishmaniasis by 2-Dimentional Electrophoresis and Mass Spectrometry, Iranian Journal of Parasitology (2015)
12. In Vitro and in Vivo Therapeutic Potentials of 6-Gingerol in Combination With Amphotericin B for Treatment of Leishmania Major Infection: Powerful Synergistic and Multifunctional Effects, International Immunopharmacology (2021)
13. Involvement of Tryparedoxin Peroxidase (Tryp) and Trypanothione Reductase (Tryr) in Antimony Unresponsive of Leishmania Tropica Clinical Isolates of Iran, Acta Tropica (2022)
14. Development of a Topical Liposomal Formulation of Amphotericin B for the Treatment of Cutaneous Leishmaniasis, International Journal for Parasitology: Drugs and Drug Resistance (2019)
15. Review of the Prevalence and Causes of Antimony Compounds Resistance in Different Societies: Review Article, Tehran University Medical Journal (2017)
16. An Immunoproteomic Approach to Identifying Immunoreactive Proteins in Leishmania Infantum Amastigotes Using Sera of Dogs Infected With Canine Visceral Leishmaniasis, Pathogens and Global Health (2019)
17. Probability of False-Negative Results in Microscopical Detection of Cutaneous Leishmaniasis: More Accurate Screening by Kdna-Pcr During Epidemiological Survey, Journal of Parasitic Diseases (2020)
18. A Novel Strategy for Enhance Potentiation of Meglumine Antimoniate Against Leishmania Major in Vitro, Iranian Journal of Parasitology (2019)
19. Comparison of P27 Gene Expression of Promastigote and Amastigote Forms of Leishmania Major (Mrho/Ir/75/Er) by Real-Time Rt-Pcr, Iranian Journal of Parasitology (2018)
20. Photodynamic Therapy Using Toluidine Blue O (Tbo) Dye As a Photosensitizer Against Leishmania Major, Iranian Journal of Public Health (2021)
21. Potent Antileishmanial Activity of Chitosan Against Iranian Strain of Leishmania Major (Mrho/Ir/75/Er): In Vitro and in Vivo Assay, Journal of Vector Borne Diseases (2018)
22. Mechanistic View for Toxic Effects of Arsenic on Isolated Rat Kidney and Brain Mitochondria, Biologia (Poland) (2015)
23. The Effects of Medicinal Herbs and Marine Natural Products on Wound Healing of Cutaneous Leishmaniasis: A Systematic Review, Microbial Pathogenesis (2021)
24. Safety Evaluation of Nano-Liposomal Formulation of Amphotericin B (Sina Ampholeish) in Animal Model As a Candidate for Treatment of Cutaneous Leishmaniasis, Journal of Arthropod-Borne Diseases (2018)
25. Quantitative Proteomic Analysis Reveals Differentially Expressed Proteins in Leishmania Major Metacyclogenesis, Microbial Pathogenesis (2020)
26. An Overview of Leishmanization Experience: A Successful Control Measure and a Tool to Evaluate Candidate Vaccines, Acta Tropica (2019)
27. Identification of Immunodominant Proteins of Leishmania Infantum by Immunoproteomics to Evaluate a Recombinant Multi-Epitope Designed Antigen for Serodiagnosis of Human Visceral Leishmaniasis, Experimental Parasitology (2021)
28. Treatment of Resistant Visceral Leishmaniasis With Interferon Gamma in Combination With Liposomal Amphotericin B and Allopurinol, Parasitology International (2019)