New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140

New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140 Publisher Pubmed

Mahboudi H¹ ; Soleimani M² ; Hanaeeahvaz H³ ; Ghanbarian H¹ ; Bandehpour M¹ ; Enderami SE³ ; Kazemi B^{1, 4}

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1. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3. Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran
4. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: ASAIO Journal Published:2018

Abstract

Mesenchymal stem cells are widely stimulated by transforming growth factor beta-3 (TGFß3) for chondrocyte differentiation. The objective of our study was to establish a new method for differentiation of human mesenchymal stem cells toward chondrocyte by overexpression of MicroRNA-140 (miR-140), and also this method was compared with method of induction with TGFß3 in high-cell density culture systems. Mesenchymal stem cells were harvested from bone marrow of human. We prepared vectors and then was used for recombinant Lenti virus production in HEK-293 cell. Transducted cells were cultured in monolayer culture system and were harvested after days 7, 14, and 21. Real time polymerase chain reaction ( RT-PCR) was performed to evaluate the cartilage-specific genes in the mRNA levels. Also, in order to confirm our results, we have done immunocytochemistry technique. Bone marrow mesenchymal stem cells (BMSCs) were transducted with recombinant Lenti virus, and miR-140 was expressed. Immunocytochemical method confirmed the differentiation of BMSC toward chondrocyte with handling cartilage matrix genes. Also real-time PCR showed that after expression of miR-140 in transducted BMSCs significantly increased gene expression of collagen type II and aggrecan and downregulated expression of collagen type I when compared with the mRNA levels measured in nontransducted BMSCs. These results were compatible compared with TGFß3 induction method as control positive. In this study, we described a new approach and technique that may be applied for differentiation of BMSCs to chondrocyte instead of stimulation with TGFß3. Our data implies that miR-140 is a potent chondrogenic differentiation inducer for BMSCs, and we have shown increasing chondrogenic differentiation by using miR-140 overexpression. ASAIO Journal 2018; 64: 662-672. Copyright © 2017 by the ASAIO.

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Style	Citing Format
MLA	Mahboudi H, et al.. "New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140." ASAIO Journal, vol. 64, no. 5, 2018, pp. 662-672.
APA	Mahboudi H, Soleimani M, Hanaeeahvaz H, Ghanbarian H, Bandehpour M, Enderami SE, Kazemi B (2018). New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140. ASAIO Journal, 64(5), 662-672.
Chicago	Mahboudi H, Soleimani M, Hanaeeahvaz H, Ghanbarian H, Bandehpour M, Enderami SE, Kazemi B. "New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140." ASAIO Journal 64, no. 5 (2018): 662-672.
Harvard	Mahboudi H et al. (2018) 'New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140', ASAIO Journal, 64(5), pp. 662-672.
Vancouver	Mahboudi H, Soleimani M, Hanaeeahvaz H, Ghanbarian H, Bandehpour M, Enderami SE, et al.. New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140. ASAIO Journal. 2018;64(5):662-672.
BibTex	@article{ author = {Mahboudi H and Soleimani M and Hanaeeahvaz H and Ghanbarian H and Bandehpour M and Enderami SE and Kazemi B}, title = {New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140}, journal = {ASAIO Journal}, volume = {64}, number = {5}, pages = {662-672}, year = {2018} }
RIS	TY - JOUR AU - Mahboudi H AU - Soleimani M AU - Hanaeeahvaz H AU - Ghanbarian H AU - Bandehpour M AU - Enderami SE AU - Kazemi B TI - New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140 JO - ASAIO Journal VL - 64 IS - 5 SP - 662 EP - 672 PY - 2018 ER -

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