Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3)

Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3) Publisher Pubmed

Mahboudi H¹ ; Soleimani M² ; Enderami SE³ ; Kehtari M³ ; Ardeshirylajimi A⁴ ; Eftekhary M¹ ; Kazemi B^{1, 5}

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1. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3. Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran
4. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Biologicals Published:2018

Abstract

Induced pluripotent stem cells (iPSCs) make an attractive source for regenerative medicine. The objective of our study was to establish a new method for differentiation of human iPSCs toward chondrocyte by overexpression of MicroRNA-140 (miR-140) and use of transforming growth factor beta 3 (TGFβ3) in high-cell density culture systems. We prepared vectors and then was used for recombinant Lenti virus production in HEK-293 cell. Transducted cells were selected and cultured in pellet culture system and were harvested after days 7, 14 and 21. Real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels. Also, in order to confirm our results, we have done immunological assay. iPSCs were transducted with recombinant Lenti virus and miR-140 was expressed. Immunological methods confirmed that differentiation of iPSC toward chondrocyte with handling cartilage matrix genes. Also real time PCR demonstrated that in transducted iPSCs significantly increased gene expression of collagen type II, SOX9 and aggrecan, and down-regulated expression of collagen type I when compared to the mRNA levels measured in non transducted iPSCs. In Conclusion, our data implies that miR-140 is a potent chondrogenic differentiation inducer for iPSCs and also, we have showed increasing chondrogenic differentiation by using overexpression of miR-140 and TGFβ3. © 2018

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Style	Citing Format
MLA	Mahboudi H, et al.. "Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3)." Biologicals, vol. 52, no. , 2018, pp. 30-36.
APA	Mahboudi H, Soleimani M, Enderami SE, Kehtari M, Ardeshirylajimi A, Eftekhary M, Kazemi B (2018). Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3). Biologicals, 52(), 30-36.
Chicago	Mahboudi H, Soleimani M, Enderami SE, Kehtari M, Ardeshirylajimi A, Eftekhary M, Kazemi B. "Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3)." Biologicals 52, no. (2018): 30-36.
Harvard	Mahboudi H et al. (2018) 'Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3)', Biologicals, 52(), pp. 30-36.
Vancouver	Mahboudi H, Soleimani M, Enderami SE, Kehtari M, Ardeshirylajimi A, Eftekhary M, et al.. Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3). Biologicals. 2018;52():30-36.
BibTex	@article{ author = {Mahboudi H and Soleimani M and Enderami SE and Kehtari M and Ardeshirylajimi A and Eftekhary M and Kazemi B}, title = {Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3)}, journal = {Biologicals}, volume = {52}, number = {}, pages = {30-36}, year = {2018} }
RIS	TY - JOUR AU - Mahboudi H AU - Soleimani M AU - Enderami SE AU - Kehtari M AU - Ardeshirylajimi A AU - Eftekhary M AU - Kazemi B TI - Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3) JO - Biologicals VL - 52 IS - SP - 30 EP - 36 PY - 2018 ER -

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