The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells

The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells Publisher Pubmed

Mahboudi H¹ ; Soleimani M² ; Enderami SE^{3, 4} ; Kehtari M⁴ ; Hanaee Ahvaz H⁴ ; Ghanbarian H¹ ; Bandehpour M¹ ; Nojehdehi S⁴ ; Mirzaei S⁴ ; Kazemi B⁵

Show Affiliations

1. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3. Cancer Gene Therapy Research Center, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
4. Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran
5. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2018

Abstract

Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. Chondrogenic differentiation of iPSCs is important for application in cartilage tissue engineering. In this study, we considered the effect of nanofibre-based polyethersulfone (PES) scaffold on the chondrogenesis of iPSCs. IPSC cells were cultured on the PES scaffold and scaffold free method. After 21 d, real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels. For confirm our results, we have done immunocytochemistry and scanning electron microscopy (SEM) imaging. According to the results, higher significant expressions of common chondrogenic-related genes such as aggrecan, collagen type II and collagen type X were observed in PES seeded human iPSCs when compared to the mRNA levels measured in scaffold free method. Expression of collagen type I down regulated in both methods. Also, both methods were showed a similar pattern of expression of SOX9. Our results showed that nanofibre-based PES scaffold enhanced the chondrogenesis of iPSCs and the highest capacity for differentiation into chondrocyte-like cells. These cells and PES scaffold were demonstrated to have great efficiency for treatment of cartilage damages and lesions. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.

Related Docs

View other Related Docs

1. Enhanced Chondrogenesis of Human Bone Marrow Mesenchymal Stem Cell (Bmsc) on Nanofiber-Based Polyethersulfone (Pes) Scaffold, Gene (2018)

2. Enhanced Chondrogenesis Differentiation of Human Induced Pluripotent Stem Cells by Microrna-140 and Transforming Growth Factor Beta 3 (Tgfβ3), Biologicals (2018)

3. Enhanced Chondrogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells on Pcl/Plga Electrospun With Different Alignments and Compositions, International Journal of Polymeric Materials and Polymeric Biomaterials (2018)

4. Poly (3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Improved Osteogenic Differentiation of the Human Induced Pluripotent Stem Cells While Considered As an Artificial Extracellular Matrix, Journal of Cellular Physiology (2019)

5. Hepatogenic Differentiation of Human Induced Pluripotent Stem Cells on Collagen-Coated Polyethersulfone Nanofibers, ASAIO Journal (2017)

6. New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of Microrna-140, ASAIO Journal (2018)

7. Collagen Coated Electrospun Polyethersulfon Nanofibers Improved Insulin Producing Cells Differentiation Potential of Human Induced Pluripotent Stem Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)

8. Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2018)

Experts (# of related papers)

Mohammad Vasei (2)

Ghodratollah Panahi (1)

Style	Citing Format
MLA	Mahboudi H, et al.. "The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells." Artificial Cells# Nanomedicine and Biotechnology, vol. 46, no. 8, 2018, pp. 1948-1956.
APA	Mahboudi H, Soleimani M, Enderami SE, Kehtari M, Hanaee Ahvaz H, Ghanbarian H, Bandehpour M, Nojehdehi S, Mirzaei S, Kazemi B (2018). The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells. Artificial Cells# Nanomedicine and Biotechnology, 46(8), 1948-1956.
Chicago	Mahboudi H, Soleimani M, Enderami SE, Kehtari M, Hanaee Ahvaz H, Ghanbarian H, Bandehpour M, Nojehdehi S, Mirzaei S, Kazemi B. "The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells." Artificial Cells# Nanomedicine and Biotechnology 46, no. 8 (2018): 1948-1956.
Harvard	Mahboudi H et al. (2018) 'The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells', Artificial Cells# Nanomedicine and Biotechnology, 46(8), pp. 1948-1956.
Vancouver	Mahboudi H, Soleimani M, Enderami SE, Kehtari M, Hanaee Ahvaz H, Ghanbarian H, et al.. The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells. Artificial Cells# Nanomedicine and Biotechnology. 2018;46(8):1948-1956.
BibTex	@article{ author = {Mahboudi H and Soleimani M and Enderami SE and Kehtari M and Hanaee Ahvaz H and Ghanbarian H and Bandehpour M and Nojehdehi S and Mirzaei S and Kazemi B}, title = {The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells}, journal = {Artificial Cells# Nanomedicine and Biotechnology}, volume = {46}, number = {8}, pages = {1948-1956}, year = {2018} }
RIS	TY - JOUR AU - Mahboudi H AU - Soleimani M AU - Enderami SE AU - Kehtari M AU - Hanaee Ahvaz H AU - Ghanbarian H AU - Bandehpour M AU - Nojehdehi S AU - Mirzaei S AU - Kazemi B TI - The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells JO - Artificial Cells# Nanomedicine and Biotechnology VL - 46 IS - 8 SP - 1948 EP - 1956 PY - 2018 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract