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Key Genes and Regulatory Networks Involved in the Initiation, Progression and Invasion of Colorectal Cancer Publisher



Asghari M1 ; Abazari MF2 ; Bokharaei H3 ; Aleagha MN2 ; Poortahmasebi V4 ; Askari H5 ; Torabinejad S2 ; Ardalan A6 ; Negaresh N7 ; Ataei A8 ; Pazooki P9 ; Poorebrahim M10
Authors
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Authors Affiliations
  1. 1. Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute of Biotechnology, ACECR, Isfahan, Iran
  2. 2. Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran
  3. 3. Department of Genetics, Faculty of Basic Sciences, Science and Research Branch, Azad University, Tehran, Iran
  4. 4. Hepatitis B Molecular Laboratory, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Biology, Faculty of Sciences, Arak University, Arak, Iran
  7. 7. Department of Medicine, Faculty of Medicine, Qom Branch, Islamic Azad University, Qom, Iran
  8. 8. Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation
  9. 9. Department of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran
  10. 10. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Future Science OA Published:2018


Abstract

Aim: Until now, identification of drug targets for treatment of patients with specific stages of colorectal cancer (CRC) has remained a challenging field of research. Herein, we aimed to identify the key genes and regulatory networks involved in each stage of CRC. Results: The results of gene expression profiles were integrated with protein-protein interaction networks, and topologically analyzed. The most important regulatory genes (e.g., CDK1, UBC, ESR1 and ATXN1) and signaling pathways (e.g., Wnt, MAPK and JAK-STAT) in CRC initiation, progression and metastasis were identified. In vitro analysis confirmed some in silico findings. Conclusion: Our study introduces functional hub genes, subnetworks, prioritizes signaling pathways and novel biomarkers in CRC that may guide further development of targeted therapy programs. A comprehensive view of the molecular mechanisms involved in colorectal cancer (CRC) pathogenesis has been demonstrated. Cell cycle and signal transduction genes were contributors to CRC initiation and progression. In contrast to stage IV, the stages I-III of CRC shared a notable number of common genes suggesting their close gene signatures. The efficacy of these predictions has been validated using two colon cancer cell lines in vitro. © 2018 Mansour Poorebrahim.
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