Single Nucleotide Polymorphisms of the Genes Encoding Il-10 and Tgf-Β1 in Iranian Children With Atopic Dermatitis Publisher Pubmed



Behniafard N^{1, 2} ; Amirzargar AA^{3, 4} ; Gharagozlou M¹ ; Delavari F^{2, 5} ; Hosseinverdi S² ; Sotoudeh S¹ ; Farhadi E⁶ ; Mahmoudi M^{7, 8} ; Khaledi M⁹ ; Moghaddam ZG¹ ; Aghamohammadi A^{1, 2} ; Rezaei N^{2, 3, 10}
Source: Allergologia et Immunopathologia Published:2018

Abstract

Background: Atopic dermatitis is an inflammatory skin disease in which both genetic and environmental factors interact to determine the susceptibility and severity of the disease. Objective: The aim of this study was to determine the association between atopic dermatitis and IL-10 and TGF-β1 gene polymorphisms. Methods: The allele and genotype frequencies of genes encoding for IL-10 and TGF-β1 were investigated in 89 patients with atopic dermatitis in comparison with 138 in the control group using the PCR-SSP method. Results: A significant increase was found in the frequency of the TGF-β1 codon 10/C allele among patients (p < 0.001, OR = 6.77), whereas a significant decrease was observed in the frequency of the T allele at the same position (p < 0.001, OR = 0.14). The frequency of the TGF-β1 codon 25/G allele in the control group was significantly higher than among patients (p < 0.001, OR = 0.08). A significant positive correlation was seen between CC (p < 0.001, OR = 15.10) and CG (p < 0.001) genotypes and AD at codons 10 and 25, respectively. The most frequent haplotypes among patients was TGF-β1 CG which was significantly higher than in the control subjects (50% in patients vs. 39.9% in controls, p = 0.042). A significant increase was found in the frequency of TGF-β CC (36% in patients vs. 7.6% in controls, p < 0.001) and TC (14% in patients vs. 0% in controls, p < 0.001) haplotypes among patients compared to controls. By contrast, the TGF-β1 TG haplotype was significantly lower in patients than controls (0% in patients vs. 52.5% in controls, p < 0.001). There were no significant differences in the frequency of alleles, genotypes and haplotypes of the IL-10 gene. Conclusions: We found a strong association between the polymorphisms of the TGF-β1 gene at codon 10 and codon 25 positions and atopic dermatitis. © 2017 SEICAP