Interleukin 10 and Transforming Growth Factor Beta Polymorphisms As Risk Factors for Kawasaki Disease: A Case-Control Study and Meta-Analysis



Rahmani F^{1, 2} ; Ziaee V^{3, 4} ; Assari R^{3, 4} ; Sadr M⁵ ; Rezaei A¹ ; Sadr Z⁵ ; Reza Raeeskarami S⁶ ; Hassan Moradinejad M⁴ ; Aghighi Y⁷ ; Rezaei N^{1, 8, 9}
Source: Avicenna Journal of Medical Biotechnology Published:2019

Abstract

Background: Alteration in serum expression of Transforming Growth Factor-beta (TGF-Β) and IL-10 have been suggested to play a role in the pathogenesis of Kawasaki Disease (KD). Inconsistent reports exist on the association of IL-10 polymorphisms with KD susceptibility and Coronary Artery Aneurysms (CAA). Methods: A number of 110 paediatric patients with KD and 140 healthy individuals were recruited to investigate the frequency of Single Nucleotide Polymorphisms (SNPs) of TGF-Β C/T at codon 10 (rs1982073), C/G at codon 25 (rs1800471) and IL-10 A/G at -1082 (rs1800896), C/T at -819 (rs1800871) and A/C at -592 (rs1800872) and their respective genotype and haplotypes. A comprehensive search was performed in MEDLINE and SCOPUS using the keywords of interleukin 10, transforming growth factor beta, and Kawasaki disease. Moreover, previous studies investigating the TGF- Β and IL-10 polymorphisms in KD were evaluated. Review Manager Version 5.1 Software was used to perform meta-analysis. Results: There was no significant association between allelic or genotypic variants in the mentioned polymorphisms in TGF-Β or IL-10 with KD or CAA. The only significant haplotypic variant was TC variant at codon 10, and 25 of TGF-Β polymorphisms were associated with higher risk of KD. Meta-analysis of a total number of 770 patients vs. 1471 healthy controls showed no difference in the frequency of any of the IL-10 genetic variants in KD patients, regardless of the presence of CAA. Conclusion: Polymorphisms of TGF-Β or IL-10 are not associated with additional risk for KD in Iranian population. IL-10 polymorphisms at -1082, -819 and -592 positions are not associated with KD, nor do they predict coronary artery aneurysm formation. © 2019, Avicenna Journal of Medical Biotechnology.