Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Synthesis of Polyethyleneimine (Pei) and Β-Cyclodextrin Grafted Pei Nanocomposites With Magnetic Cores for Lipase Immobilization and Esterification Publisher



Khoobi M1 ; Khalilvandsedagheh M2 ; Ramazani A2 ; Asadgol Z1 ; Forootanfar H3 ; Faramarzi MA1, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, 1417614411, Iran
  2. 2. Department of Chemistry, University of Zanjan, Zanjan, Iran
  3. 3. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
  4. 4. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 1417614411, Iran

Source: Journal of Chemical Technology and Biotechnology Published:2016


Abstract

BACKGROUND: Fabrication of nanocomposites with a magnetic core and their applications for enzyme immobilization has been introduced as an intresting trend during recent decades. The present study described the synthesis of polyethyleneimine (PEI) and β-cyclodextrin grafted PEI nanocomposites with magnetic cores and their applications for lipase immobilization. Fe3O4 magnetic nanoparticles (MNPs) were prepared by co-precipitation of Fe (III) and Fe (II) ions and then coated with polyethylenimine and/or hydroxyapatite/polyethylenimine, followed by grafting β-cyclodextrin onto the surface of the prepared nanocomposites. The nanocomposites were then characterized using Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, vibrating sample magnetometry, and scanning and transmission electron microscopy and consequently applied for the immobilization of lipase. RESULTS: The results obtained for thermal and pH stability, reusability, and storage stability showed that the MNPs at hydroxyapatite/polyethylenimine/β-cyclodextrin was the best nanocomposite for lipase immobilization. Application of the immobilized lipase onto the selected nanocomposite for the synthesis of ethyl valerate (green apple flavor) revealed esterification rates of 79% and 62% in n-hexane and dimethyl sulfoxide, respectively, after 24 h incubation. CONCLUSION: The results obtained demonstrate good stability, reusability, and potential application of immobilized lipase on magnetic nanocomposite of MNPs at hydroxyapatite/polyethylenimine/β-cyclodextrin for synthesis of ethyl valerate especially in organic medium. © 2014 Society of Chemical Industry © 2016 Society of Chemical Industry.
Related Docs
View other Related Docs
1. Metal-Chelate Immobilization of Lipase Onto Polyethylenimine Coated Mcm-41 for Apple Flavor Synthesis, Applied Biochemistry and Biotechnology (2017)
2. Insights Into Laccase Producing Organisms, Fermentation States, Purification Strategies, and Biotechnological Applications, Biotechnology Progress (2015)
3. Immobilization of Thermoalkalophilic Lipase From Bacillus Atrophaeus Fshm2 on Amine-Modified Graphene Oxide Nanostructures: Statistical Optimization and Its Application for Pentyl Valerate Synthesis, Applied Biochemistry and Biotechnology (2020)
4. Immobilization of Lipase on the Modified Magnetic Diatomite Earth for Effective Methyl Esterification of Isoamyl Alcohol to Synthesize Banana Flavor, 3 Biotech (2020)
5. Elimination and Detoxification of Sulfathiazole and Sulfamethoxazole Assisted by Laccase Immobilized on Porous Silica Beads, International Biodeterioration and Biodegradation (2015)
6. Recent Developments in the Fungal Transformation of Steroids, Biocatalysis and Biotransformation (2015)
7. Efficient Decolorization and Detoxification of Reactive Orange 7 Using Laccase Isolated From Paraconiothyrium Variabile, Kinetics and Energetics, Journal of the Taiwan Institute of Chemical Engineers (2015)
8. Laccase-Mediated Treatment of Pharmaceutical Wastes, Research Advancements in Pharmaceutical# Nutritional# and Industrial Enzymology (2018)
Experts (# of related papers)
View all Related Experts
Mohammad Ali Faramarzi (23)
Mehdi Khoobi (6)
Other Related Docs
9. Optimization of Immobilization Conditions of Bacillus Atrophaeus Fshm2 Lipase on Maleic Copolymer Coated Amine-Modified Graphene Oxide Nanosheets and Its Application for Valeric Acid Esterification, International Journal of Biological Macromolecules (2020)
10. Biocatalytic Conversion and Detoxification of Imipramine by the Laccase-Mediated System, International Biodeterioration and Biodegradation (2016)
11. Thermoalkalophilic Lipase From an Extremely Halophilic Bacterial Strain Bacillus Atrophaeus Fshm2: Purification, Biochemical Characterization and Application, Biocatalysis and Biotransformation (2017)
12. Studies on the Laccase-Mediated Decolorization, Kinetic, and Microtoxicity of Some Synthetic Azo Dyes, Journal of Environmental Health Science and Engineering (2016)
13. Metal Nanoparticles in Atherosclerosis: Applications and Potential Toxicity, Nano Biomedicine and Engineering (2015)
14. Application of Free and Immobilized Laccase for Removal and Detoxification of Fluoroquinolones From Aqueous Solution, Global Nest Journal (2020)
15. Magnetic Casein Aggregates As an Innovative Support Platform for Laccase Immobilization and Bioremoval of Crystal Violet, International Journal of Biological Macromolecules (2022)
16. Isolation, One-Step Affinity Purification, and Characterization of a Polyextremotolerant Laccase From the Halophilic Bacterium Aquisalibacillus Elongatus and Its Application in the Delignification of Sugar Beet Pulp, Bioresource Technology (2017)
17. Functionalized Magnetic Nanoparticles for the Separation and Purification of Proteins and Peptides, TrAC - Trends in Analytical Chemistry (2021)
18. Evaluation of a Novel Biocompatible Magnetic Nanomedicine Based on Beta-Cyclodextrin, Loaded Doxorubicin-Curcumin for Overcoming Chemoresistance in Breast Cancer, Artificial Cells# Nanomedicine and Biotechnology (2018)
19. Optimization of the Enzymatic Elimination of Flumequine by Laccase-Mediated System Using Response Surface Methodology, Desalination and Water Treatment (2016)
20. Comparative Study of in Vitro Prooxidative Properties and Genotoxicity Induced by Aflatoxin B1 and Its Laccase-Mediated Detoxification Products, Chemosphere (2015)
21. Biomedical and Pharmaceutical-Related Applications of Laccases, Current Protein and Peptide Science (2020)
22. Polyoxometalate-Metal Organic Framework-Lipase: An Efficient Green Catalyst for Synthesis of Benzyl Cinnamate by Enzymatic Esterification of Cinnamic Acid, International Journal of Biological Macromolecules (2018)
23. Statistical Optimization of Cultural Medium Composition of Thermoalkalophilic Lipase Produced by a Chemically Induced Mutant Strain of Bacillus Atrophaeus Fshm2, 3 Biotech (2019)
24. Laccase-Catalyzed Decolorization and Detoxification of Acid Blue 92: Statistical Optimization, Microtoxicity, Kinetics, and Energetics, Journal of Environmental Health Science and Engineering (2015)
25. Laccase-Catalyzed Treatment of Ketoconazole, Identification of Biotransformed Metabolites, Determination of Kinetic Parameters, and Evaluation of Micro-Toxicity, Journal of Molecular Catalysis B: Enzymatic (2016)
26. Graphene-Based Polymer Nanocomposites: Chemistry and Applications, Advanced Structured Materials (2015)