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Comparative Study of in Vitro Prooxidative Properties and Genotoxicity Induced by Aflatoxin B1 and Its Laccase-Mediated Detoxification Products Publisher Pubmed



Zeinvandlorestani H1 ; Sabzevari O1 ; Setayesh N2 ; Amini M3 ; Niliahmadabadi A4 ; Faramarzi MA2
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Authors Affiliations
  1. 1. Department of Pharmacology and Toxicology, Faculty of Pharmacy and Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, 1417614411, Iran
  2. 2. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, 1417614411, Iran
  3. 3. Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran

Source: Chemosphere Published:2015


Abstract

In this paper, the enzymatic detoxification of aflatoxin B1 (AFB1) by laccase was studied, and the prooxidant properties and mutagenicity of the detoxification products were compared with those of AFB1. The optimal enzymatic reaction occurred in 0.1M of citrate buffer containing 20% DMSO at 35°C, a pH of 4.5, and a laccase activity of 30UmL-1. After 2d, sixty-seven percent of the toxic substrate was removed. The prooxidative properties of the detoxified products (27% versus 86%) and the mutagenicity were significantly decreased in comparison with the parent toxin. Unlike AFB1, which promoted metabolism-dependent genetic mutations by base-pair substitution, the detoxified products did not induce genotoxicity. Comparison of the Km values for AFB1 and riboflavin, a valuable food nutrient, indicated that laccase showed greater affinity for the toxin than for riboflavin. © 2015 Elsevier Ltd.
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