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Fluorometric Determination of Microrna Via Fret Between Silver Nanoclusters and Cdte Quantum Dots Publisher



Borghei YS1 ; Hosseini M1, 2 ; Ganjali MR3, 4
Authors
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Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, 1417466191, Iran
  2. 2. Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
  4. 4. Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Microchimica Acta Published:2017


Abstract

This paper describes a CdTe quantum dot-based fluorescence resonance energy transfer (FRET) based assay for the detection of the breast cancer biomarker microRNA. The method relies on energy transfer between DNA-templated silver nanoclusters (AgNCs) and CdTe QDs. Interaction between double strand oligonucleotide and QDs can be detected qualitatively through gel analysis and quantitatively by the signal amplification from AgNCs to QDs via FRET, best measured at an excitation wavelength of 350 nm and at emission wavelengths of 550 and 590 nm. Three microRNAs (microRNA-21, microRNA-155 and Let-7a) were quantified to verify the feasibility of the method, and a high sensitivity for microRNAs was achieved. Fluorescence intensity increases linearly with the log of the concentration of microRNA 155 in the 5.0 pM to 50 nM range, with a 1.2 pM detection limit. [Figure not available: see fulltext.]. © 2017, Springer-Verlag GmbH Austria.
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