Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Visual Detection of Mirna Using Peroxidase-Like Catalytic Activity of Dna-Cuncs and Methylene Blue As Indicator Publisher Pubmed



Borghei YS1 ; Hosseini M1, 2 ; Ganjali MR3, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran
  2. 2. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran
  4. 4. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Clinica Chimica Acta Published:2018


Abstract

In this study, we developed a colorimetric method which is primarily based on the limited amount of redox in response to the miRNA target analyte binding for the determination of the miRNA, which is used as a biomarker to confirm the clinical diagnosis of cancers such as breast cancer. This method is on the basis of the peroxidase-like property of DNA templated copper nanoclusters (DNA-CuNCs). In this research, it was found that the DNA (poly T) stabilized green emitting CuNCs which exhibited enzyme-like peroxidase activity. After hybridization of miRNA with DNA-CuNCs probe and duplex formation, CuNCs catalyzed the oxidation of the methylene blue (MB) substrate, which can interact easily with DNA/miR-155 heteroduplex. Applying the optimal conditions, the absorbance of MB decreased by increasing the target miRNA-155 with in a dynamic range from 1.0 pM to 10.0 nM down to a detection limit (LOD) of 0.6 pM. The DNA-CuNCs/MB complex was designed to develop a facile, cheap, and fast colorimetric assay for detection of miRNA through MB oxidation by DNA stabilized CuNCs. © 2018 Elsevier B.V.
Related Docs
View other Related Docs
1. Colorimetric and Energy Transfer Based Fluorometric Turn-On Method for Determination of Microrna Using Silver Nanoclusters and Gold Nanoparticles, Microchimica Acta (2018)
2. Fluorometric Determination of Microrna Via Fret Between Silver Nanoclusters and Cdte Quantum Dots, Microchimica Acta (2017)
3. A Fluorometric Study on the Effect of Dna Methylation on Dna Interaction With Graphene Quantum Dots, Methods and Applications in Fluorescence (2019)
4. Fluorescence Based Turn-On Strategy for Determination of Microrna-155 Using Dna-Templated Copper Nanoclusters, Microchimica Acta (2017)
5. Oxidase-Like Catalytic Activity of Cys-Auncs Upon Visible Light Irradiation and Its Application for Visual Mirna Detection, Sensors and Actuators# B: Chemical (2018)
6. Detection of Large Deletion in Human Brca1 Gene in Human Breast Carcinoma Mcf-7 Cells by Using Dna-Silver Nanoclusters, Methods and Applications in Fluorescence (2018)
7. Synthesis of Fluorescent Cysteine-Gold Nano-Clusters (Cys-Au-Ncs) and Their Application As Nano-Biosensors for the Determination of Cysteine, Current Nanoscience (2017)
8. Paper Based Colorimetric Detection of Mirna-21 Using Ag/Pt Nanoclusters, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2020)
Experts (# of related papers)
Mehdi Khoobi (1)
Other Related Docs
9. A Unique Fret Approach Toward Detection of Single-Base Mismatch Dna in Brca1 Gene, Materials Science and Engineering C (2019)
10. A Colorimetric Paper Sensor for Citrate As Biomarker for Early Stage Detection of Prostate Cancer Based on Peroxidase-Like Activity of Cysteine-Capped Gold Nanoclusters, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2019)
11. Sensitive Detection of S. Aureus Using Aptamer- and Vancomycin -Copper Nanoclusters As Dual Recognition Strategy, Food Chemistry (2021)
12. A Novel Brca1 Gene Deletion Detection in Human Breast Carcinoma Mcf-7 Cells Through Fret Between Quantum Dots and Silver Nanoclusters, Journal of Pharmaceutical and Biomedical Analysis (2018)
13. Label-Free Fluorescent Detection of Microrna-155 Based on Synthesis of Hairpin Dna-Templated Copper Nanoclusters by Etching (Top-Down Approach), Sensors and Actuators# B: Chemical (2017)
14. Biomarker Sensing Using Luminescent Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
15. New Insight Into G-Quadruplexes; Diagnosis Application in Cancer, Analytical Biochemistry (2021)
16. A New Eye Dual-Readout Method for Mirna Detection Based on Dissolution of Gold Nanoparticles Via Lspr by Cdte Qds Photoinduction, Scientific Reports (2019)
17. A Label-Free Luminescent Light Switching System for Mirna Detection Based on Two Color Quantum Dots, Journal of Photochemistry and Photobiology A: Chemistry (2020)
18. Naked-Eye Detection of Potassium Ions in a Novel Gold Nanoparticle Aggregation-Based Aptasensor, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2018)
19. An Innovative Paradigm of Methods in Micrornas Detection: Highlighting Dnazymes, the Illuminators, Biosensors and Bioelectronics (2018)
20. Colorimetric Aptasensor for Campylobacter Jejuni Cells by Exploiting the Peroxidase Like Activity of Au@Pd Nanoparticles, Microchimica Acta (2018)
21. Novel Fluorometric Assay for Detection of Cysteine As a Reducing Agent and Template in Formation of Copper Nanoclusters, Journal of Fluorescence (2017)
22. Biosensing Using Dna-Based Structures Integrated With Nanosheets, Microchemical Journal (2023)
23. Colorimetric and Fluorescent Dual-Biosensor Based on Zirconium and Preasodium Metal-Organic Framework (Zr/Pr Mof) for Mirna-191 Detection, Heliyon (2024)
24. Fluorescent Turn on Sensing of Caffeine in Food Sample Based on Sulfur-Doped Carbon Quantum Dots and Optimization of Process Parameters Through Response Surface Methodology, Sensors and Actuators# B: Chemical (2018)
25. Detection of P53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters, Journal of Fluorescence (2017)
26. Fast and Selective Whole Cell Detection of Staphylococcus Aureus Bacteria in Food Samples by Paper Based Colorimetric Nanobiosensor Using Peroxidase-Like Catalytic Activity of Dna-Au/Pt Bimetallic Nanoclusters, Microchemical Journal (2020)
27. Gold Nanorod Etching for Sensitive Aptamer-Mediated Colorimetric Detection of Escherichia Coli in Water, Microchemical Journal (2025)
28. Fluorescence Self-Assembled Dna Hydrogel for the Determination of Prostate Specific Antigen by Aggregation Induced Emission, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2023)
29. A Colorimetric Assay of Dna Methyltransferase Activity Based on Peroxidase Mimicking of Dna Template Ag/Pt Bimetallic Nanoclusters, Analytical and Bioanalytical Chemistry (2018)
30. A Novel Dual-Mode and Label-Free Aptasensor Based Methodology for Breast Cancer Tissue Marker Targeting, Sensors and Actuators# B: Chemical (2020)
31. Sensitive Colorimetric Aptasensor Based on G-C3n4@Cu2o Composites for Detection of Salmonella Typhimurium in Food and Water, Microchimica Acta (2021)
32. Turn –On Fret-Based Cysteine Sensor by Sulfur-Doped Carbon Dots and Au Nanoparticles Decorated Ws2 Nanosheet, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2022)
33. Environmental Applications of Luminescent Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
34. Colorimetric Biosensor for Phenylalanine Detection Based on a Paper Using Gold Nanoparticles for Phenylketonuria Diagnosis, Microchemical Journal (2021)
35. Sensing of Molecules by Electrocatalysis Based on Green Synthesis of Nanomaterials, Topics in Catalysis (2025)
36. A Luminescence Nanosensor for Ornidazole Detection Using Graphene Quantum Dots Entrapped in Silica Molecular Imprinted Polymer, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2019)
37. Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine, ACS Omega (2023)
38. Application of Intercalating Molecules in Detection of Methylated Dna in the Presence of Silver Ions, Methods and Applications in Fluorescence (2019)