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A Doxycycline-Loaded Microfiber of Poly-Metformin/Pcl for Eradicating Melanoma Stem Cells Publisher Pubmed



Zarei B1 ; Akrami M2, 3 ; Rezaei N4 ; Mahdavi M5 ; Kamankesh M6 ; Haririan I2, 3, 7 ; Asadi M8 ; Navaeinigjeh M9
Authors
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Authors Affiliations
  1. 1. School of Pharmacy, International Campus, Tehran University of Medical Sciences Tehran, Iran
  2. 2. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Institute of Biomaterials, University of Tehran & Tehran University of Medical Sciences (IBUTUMS), Tehran, Iran
  4. 4. Department of Biotechnology, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
  5. 5. Endocrinology & Metabolism Research Institute, Tehran University of Medical sciences, Tehran, Iran
  6. 6. Department of Polymer Chemistry, School of Chemistry, College of Science, University of Tehran, Iran
  7. 7. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Medicinal Chemistry, Faculty of Pharmacy, Iran University of Medical Sciences, Tehran, Iran
  9. 9. Pharmaceutical Sciences Research Center (PSRC), Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Pharmaceutics Published:2024


Abstract

Nowadays, electrospun fibrous mats are used as drug delivery systems for loading of potential drugs in order to kill cancer cells. In the study, a skin patch for treating melanoma cancer after surgery was made using polycaprolactone and polymetformin microfibers that were loaded with doxycycline (PolyMet/PCL@DOX), an anti-cancer stem cell agent. The morphology, structure, mechanical characteristics, swelling, and porosity of the electrospun microfibers were examined. Drug release and anticancer effectiveness of PolyMet/PCL@DOX was evaluated against A375 melanoma cancer stem cells using the MTS, Flow cytometry, colony formation and CD44 expression assays. Scanning electron microscopy (SEM) verified the micro fibrous structure with a diameter of about 2.31 µm. The porosity and swelling percentages for microfibers was 73.5 % and 2.9 %, respectively. The tensile strength at the breaking point was equal to 3.84 MPa. The IC50 of PolyMet/PCL@DOX was 7.4 μg/mL. The survival rate of A375 cells after 72 h of PolyMet/PCL@DOX treatment was 43.9 %. The colony formation capacity of A375 cells decreased after PolyMet/PCL@DOX treatment. The level of CD44 expression in the PolyMet/PCL@DOX group decreased compared to the control group. Generally, PolyMet/PCL@DOX microfibers can be a promising candidate as a patch after surgery to eradicate cancer stem cells, effectively. © 2024
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