In Vitro Activities of Five Antifungal Agents Against 199 Clinical and Environmental Isolates of Aspergillus Flavus, an Opportunistic Fungal Pathogen Publisher Pubmed



Khodavaisy S^{1, 2} ; Badali H³ ; Hashemi SJ² ; Aala F¹ ; Nazeri M⁴ ; Nouripoursisakht S⁵ ; Sorkherizi MS⁶ ; Amirizad K⁷ ; Aslani N⁷ ; Rezaie S² Show Affiliations
Source: Journal de Mycologie Medicale Published:2016

Abstract

Aspergillus flavus is the second leading cause of invasive and non-invasive aspergillosis, as well as the most common cause of fungal sinusitis, cutaneous infections, and endophthalmitis in tropical countries. Since resistance to antifungal agents has been observed in patients, susceptibility testing is helpful in defining the activity spectrum of antifungals and determining the appropriate drug for treatment. A collection of 199 clinical and environmental strains of Aspergillus flavus consisted of clinical (n = 171) and environmental (n = 28) were verified by DNA sequencing of the partial b-tubulin gene. MICs of amphotericin B, itraconazole, voriconazole, posaconazole, and MEC of caspofungin were determined in accordance with the Clinical and Laboratory Standards Institute M38-A2 document. Caspofungin, followed by posaconazole, exhibited the lowest minimum inhibitory concentrations (MIC). All isolates had caspofungin MEC90 (0.063 μg/ml) lower than the epidemiologic cutoff values, and 3.5% of the isolates had amphotericin B MIC higher than the epidemiologic cutoff values. However, their clinical effectiveness in the treatment of A. flavus infection remains to be determined. © 2016 Elsevier Masson SAS.