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Expression Levels of Foxo-1, P27kip1, Mir-27, Mir-186 and Akt1/Akt-P Proteins in Women With Endometrial Cancer and Hyperplasia: Implications for the Human Reproductive System Publisher



Ghaderi P1 ; Fallah S2 ; Khaledi HR3 ; Tehranian A4 ; Rahmati F1 ; Sheikhhasani S5
Authors
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Authors Affiliations
  1. 1. Dept. of Biochemistry, Faculty of Biological Sciences, North-Tehran Branch, Islamic Azad University, Tehran, Iran
  2. 2. Dept. of Biochemistry Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Dept. of Agricultural Engineering, Faculty of Technical-Engineering and Basic Sciences, Shahre Rey Branch, Islamic Azad University, Tehran, Iran
  4. 4. Dept. of Obstetrics and Gynecology, Research Development Center, Arash Women׳s Hospital, Tehran, Iran
  5. 5. Obstetrician & Gynecologist – Oncologist, Tehran University of Medical Sciences, Tehran, Iran

Source: Acta Endocrinologica Published:2023


Abstract

Objectives. Despite extensive research on endometrial cancer (EC) and endometrial hyperplasia, there is still a gap in understanding the molecular mechanisms underlying their development and progression. The aim of this study was to investigate the expression levels of FOXO-1, P27Kip1, miR-27, and miR-186, and Akt1, Akt-P proteins in patients with EC and endometrial hyperplasia compared to control subjects. Subjects and methods. Samples of the endometrial tumor (n=30), normal (control) (n=30) and endometrial hyperplastic (n=30) tissue were obtained from patients referring to Arash and Imam Khomani hospitals, Tehran, Iran. Expression levels of genes and microRNAs were evaluated by qRT-PCR. Western blot analysis was applied for protein evaluation. The data were analyzed using t-test, Mann-Whitney U, Pearson correlation coefficient analysis, ANCOVA and ANOVA. Results. There was significant decrease in FOXO-1 in EC tissue compared to control tissue (p<0.05). Significant increase was observed in expression of miR-27 in patients with EC (p<0.001) and hyperplasia (p<0.01), whereas miR-186 expression level increased significantly only in patients with EC (p<0.05). P27Kip1 expression level did not significantly change in patients with EC and hyperplasia. There was a significant association between expression levels of miR-27 with FOXO-1 and P27Kip1 in patients with EC. Western blot analysis revealed higher endometrial AKT1-P protein levels in patients with EC and hyperplasia than control subjects (p<0.05). Conclusions. Our findings suggest that FOXO-1, miR-27, miR-186, and Akt1-P/Akt1 protein have the potential to serve as tissue biomarkers for early diagnosis, prognosis, and progression of EC in the human reproductive system. © 2023, Acta Endocrinologica Foundation. All rights reserved.