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Differentiation of Human Endometrial Stem Cells Into Schwann Cells in Fibrin Hydrogel As 3D Culture Publisher Pubmed



Bayat N1 ; Ebrahimibarough S2 ; Ardakan MMM2 ; Ai A3 ; Kamyab A4 ; Babaloo N2 ; Ai J1, 2
Authors
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Authors Affiliations
  1. 1. Brain and Spinal Cord Injury Research Center (BASIR), Tehran University of Medical Sciences, Keshavarz Boulevard, Gharib Street, Tehran, 6114185, Iran
  2. 2. Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran

Source: Molecular Neurobiology Published:2016


Abstract

Human endometrial stem cells (hEnSCs) are a new source of adult multipotent stem cells with the ability of differentiation into many cell lineages. Many stem cell sources are desirable for differentiation into Schwann cells. Schwann-like cells derived from hEnSCs may be one of the ideal alternative cell sources for Schwann cell generation. In this study, for differentiation of hEnSCs into Schwann cells, hEnSCs were induced with RA/FSK/PDGF-AA/HRG as an induction medium for 14 days. The cells were cultured in a tissue culture plate (TCP) and fibrin gel matrix. The viability of cultured cells in the fibrin gel and TCP was analyzed with 3-[4,5-dimethyl-2-thia-zolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay for 7 days. The attachment of cells was analyzed with SEM and DAPI staining. The expression of S100 and P75 as Schwann cell markers was evaluated by immunocytochemistry and quantitative real-time PCR (RT-PCR). The evaluation of the MTT assay and gene expression showed that the survival rate and differentiation of hEnSCs into Schwann cells in the fibrin gel were better than those in the TCP group. These results suggest that human EnSCs can be differentiated into Schwann cells in the fibrin gel better than in the TCP, and the fibrin gel might provide a suitable three-dimensional (3D) scaffold for clinical applications for cell therapy of the nervous system. © 2015, Springer Science+Business Media New York.
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