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Differentiation of Human Endometrial Stem Cells Into Endothelial-Like Cells on Gelatin/Chitosan/Bioglass Nanofibrous Scaffolds Publisher Pubmed



Shamosi A1 ; Mehrabani D2, 3 ; Azami M1 ; Ebrahimibarough S1 ; Siavashi V4 ; Ghanbari H5 ; Sharifi E1 ; Roozafzoon R1 ; Ai J1, 6
Authors
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Authors Affiliations
  1. 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  3. 3. Department of Regenerative Medicine, University of Manitoba, Winnipeg, Canada
  4. 4. Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  5. 5. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Brain and Spinal Injury Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2016


Abstract

The capacity of gelatin/chitosan/bioactive glass nanopowders (GEL/CS/BGNPs) scaffolds was investigated for increasing human endometrial stem cells (hEnSCs) differentiation into the endothelial cells in the presence of angiogenic factors. GEL/CS nanofibrous scaffold with different contents of BGNPs were fabricated and assessed. Expression of endothelial markers (CD31, vascular endothelial cadherin (VE-cadherin), and KDR) in differentiated cells was evaluated. Results showed the diameter of nanofiber increases with decreasing the BG content in GEL/CS scaffolds. Moreover, in vitro study indicated that the GEL/CS/BGNPs scaffold with 1.5% BGNPs content provided a suitable three-dimensional structure for endothelial cells differentiation. Thus, the GEL/CS/BGNPs scaffold can be recommended for blood vessels repair. © 2016 Taylor & Francis.
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