The Effects Lactobacillus Crispatus Probiotics on Proliferation and Metastasis of Cervical Cancer Cell Line Using 3D Cell Culture Publisher



Adnan A^{1, 2} ; Motevaseli E³ ; Sadroddiny E^{1, 2} Show Affiliations
Source: Indian Journal of Public Health Research and Development Published:2018

Abstract

Objective: Probiotics has been tried as an adjuvant therapy for malignant tumors through their abilities to modulate intestinal microbiota, host immune response and gene expression. Further, there are emerging data supporting the role of probiotic lactic acid bacteria in prevention of early stages colon cancer development but data on their effect on advanced colorectal cancer and cervical cancer are limited. Therefore, the aim of this study was to evaluate the impact of lactobacillus crispatus supernatant (LCS) on the human cervical cell lines (HeLa) proliferation and metastasis. Materials and Method: Effects of Lactobacillus supernatant on cell invasion in vitro was assessed by Transwell migration and invasion assays (3-D culture). The cytotoxic activity of Lactobacillus crispatus supernatant were determined by MTT assay and compared to controls, De Man Rogosa Sharpe (MRS) media and MRS + lactic acid groups. Expression of Matrix Metalloproteinase-2 (MMP2) and-9 (MMP9) genes was performed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) following the cell synchronization. Results: Lactobacillus crispatus supernatant had cytotoxic and anti-metastatic effect on HeLa but not on normal cells. Down-regulation of MMP2 and MMP9 genes expression was also observed post-LCS treatment as compared to controls, De Man Rogosa Sharpe (MRS) media and MRS + lactic acid groups (P < 0.05). Conclusion: Lactobacillus crispatus Supernatant could be a potential therapeutic agent for the treatment of advanced human cervical cancer through cytotoxic and anti-metastatic mechanisms. © 2018, Indian Journal of Public Health Research and Development. All rights reserved.