Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis

Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis Publisher

Sharifiaghdam Z¹ ; Amini SM² ; Dalouchi F¹ ; Behrooz AB^{3, 4} ; Azizi Y^{1, 5}

Show Affiliations

1. Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2. Radiation Biology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
3. Nanobiotechnology Research Group, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang, 43400, Malaysia
4. Electrophysiology Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran
5. Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran

Source: Heliyon Published:2023

Abstract

Aims: Cardiotoxicity is associated with doxorubicin (DOX), an effective anticancer drug. Apigenin has cardioprotective properties; it may be employed as a capping and reducing agent in synthesizing gold nanoparticles (AuNPs). This study examined the cardioprotective impact of AuNPs synthesized with apigenin (Api) in DOX-induced cardiotoxicity (DIC). Main methods: Api-AuNPs were synthesized in a single pot without needing additional reagents for reducing gold ions or stabilizing the NPs. The cytotoxicity of Api-AuNPs on H9c2 heart cells was subsequently determined using the MTT assay. In the animal investigation, 40 male rats were randomly assigned to one of four groups: control, cardiotoxicity (DOX), DOX treated with apigenin (DOX + Api), or DOX treated with Api-AuNPs (DOX + Api-AuNPs). To examine heart function, echocardiography was conducted. Blood samples were obtained to evaluate injury indicators (Lactate dehydrogenase (LDH), creatine kinase MB (CK-MB), Cardiac Troponin I (cTn-I), Alanine transaminase (ALT), and Aspartate transaminase (AST)). The heart was removed under general anesthetic, weighed, and preserved in formalin solution. Six micrometer-thick cardiac tissue sections were stained with hematoxylin, eosin (H&E), and immunohistochemistry to identify cardiomyocyte apoptotic markers (Bax, Bcl-2, and caspase3). Key findings: Api-AuNPs have an average size of 21.4 ± 11.6 nm and are stable in physiological environments. Api-AuNPs therapy substantially reduced body and heart weight loss compared to the DOX group. Injury indicators were reduced dramatically by Api-AuNPs treatment. Api-AuNPs inhibited myocardial apoptosis via modulating Bax, caspase3, and Bcl-2 and ameliorating tissue damage caused by DOX. Significance: Api-AuNPs' anti-apoptotic activities provide cardioprotection against DIC. It has the potential to reduce cardiotoxicity and boost myocardial performance. © 2023 The Authors

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Style	Citing Format
MLA	Sharifiaghdam Z, et al.. "Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis." Heliyon, vol. 9, no. 3, 2023, pp. -.
APA	Sharifiaghdam Z, Amini SM, Dalouchi F, Behrooz AB, Azizi Y (2023). Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis. Heliyon, 9(3), -.
Chicago	Sharifiaghdam Z, Amini SM, Dalouchi F, Behrooz AB, Azizi Y. "Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis." Heliyon 9, no. 3 (2023): -.
Harvard	Sharifiaghdam Z et al. (2023) 'Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis', Heliyon, 9(3), pp. -.
Vancouver	Sharifiaghdam Z, Amini SM, Dalouchi F, Behrooz AB, Azizi Y. Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis. Heliyon. 2023;9(3):-.
BibTex	@article{ author = {Sharifiaghdam Z and Amini SM and Dalouchi F and Behrooz AB and Azizi Y}, title = {Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis}, journal = {Heliyon}, volume = {9}, number = {3}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Sharifiaghdam Z AU - Amini SM AU - Dalouchi F AU - Behrooz AB AU - Azizi Y TI - Apigenin-Coated Gold Nanoparticles As a Cardioprotective Strategy Against Doxorubicin-Induced Cardiotoxicity in Male Rats Via Reducing Apoptosis JO - Heliyon VL - 9 IS - 3 SP - EP - PY - 2023 ER -

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