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The Effect of Antimicrobial Photodynamic Therapy Using Different Concentrations of Phycocyanin Against Streptococcus Mutans Publisher Pubmed



Abbasianfar Z1 ; Seraj B2 ; Afrasiabi S3 ; Fard MJK4 ; Ghadimi S2 ; Chiniforush N5
Authors
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Authors Affiliations
  1. 1. School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Dentofacial Deformities Research Center, Research Institute for Dental Sciences, Shahid Beheshti University of Medical Sciences, Danshjoo BLVD, Velenjak, Shahid Chamran Highway, Tehran, 19839-63113, Iran

Source: Photodiagnosis and Photodynamic Therapy Published:2024


Abstract

Background: The aim of this study is to investigate the effect of antimicrobial photodynamic therapy (aPDT) using three different concentrations of phycocyanin and two different laser powers on Streptococcus mutans. Materials and methods: In this study, Streptococcus mutans was investigated using three different concentrations of phycocyanin (2.5, 5, and 10 mg/mL), in the presence and absence of 635 nm diode laser at two power levels (400 and 500 mW). The treatments were as follows: the control group received no treatment. The next 3 groups received only the phycocyanin at 2.5, 5, and 10 mg/mL. The remaining groups received both phycocyanin and laser treatment. Finally, bacteria were counted using the colony forming units (CFUs)/mL method. Data analysis was performed using IBM SPSS 26 software and ANOVA statistical test. Results: All groups except for the phycocyanin group at a concentration of 10 mg/mL showed a statistically significant difference compared to the control group (p-Value < 0.05). Additionally, the least effect was observed in the control and the phycocyanin at a concentration of 10 mg/mL groups, while the greatest effect was found in the group receiving phycocyanin at 2.5 mg/mL concentration and laser with a power of 500 mW (2.5 + DL500). Conclusion: aPDT can be used as a complementary and non-invasive method to reduce bacterial load. The effectiveness of aPDT decreased with increasing phycocyanin concentration. Moreover, higher laser irradiation power resulted in reduced Streptococcus mutans bacterial load. Thus, the most pronounced effects were observed in the group receiving phycocyanin at 2.5 mg/mL concentration and 635 nm diode laser with a power of 500 mW (2.5 + DL500). © 2024 The Author(s)
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