Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
In Vitro Efficacy of Antimicrobial Photodynamic Therapy With Phycocyanin and Diode Laser for the Reduction of Porphyromonas Gingivalis Publisher



Etemadi A1, 2 ; Azizi A3 ; Pourhajibagher M4 ; Chiniforush N5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  2. 2. Laser Research Center of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  4. 4. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Lasers in Medical Sciences Published:2022


Abstract

Introduction: Implant surface disinfection is the most difficult phases in treatment of periimplantitis. This study sought to assess the efficacy of antimicrobial photodynamic therapy (aPDT) with phycocyanin and diode laser for the reduction of Porphyromonas gingivalis. Methods: In this in vitro, experimental study, first the minimum inhibitory concentration (MIC) of phycocyanin, the sub-lethal exposure time of the diode laser, and the sub-lethal dose of aPDT were determined. The wells containing P. gingivalis suspension were randomly divided into three experimental groups for exposure to ½ MIC phycocyanin for 5 minutes, minimum lethal dose of diode laser (635 nm, 4 minutes), and aPDT with phycocyanin and diode laser. The positive control group was exposed to 0.2% chlorhexidine (CHX) for 5 minutes, and the negative control group received no treatment. The colony count was calculated in the five groups and compared using one-way ANOVA and t test. Results: aPDT with a diode laser (635 nm, 4 minutes) and 125 μg/mL phycocyanin caused a significantly greater reduction in P. gingivalis count (mean reduction of 44.24%) compared with other groups (P < 0.0001). Minimum and maximum colony counts were noted in 0.2% CHX and negative control groups respectively. The reduction in the colony count was significant in all experimental groups, compared with the control group (P < 0.0001). Conclusion: aPDT with 635-nm diode laser and phycocyanin can significantly decrease P. gingivalis count in vitro. Considering the conservative nature of this modality, it may be used for the decontamination of peri-implant © 2022, Journal of Lasers in Medical Sciences.All Rights Reserved.
Related Docs
View other Related Docs
1. In Vitro Effect of Antimicrobial Photodynamic Therapy With Phycocyanin on Aggregatibacter Actinomycetemcomitans Biofilm on Sla Titanium Discs, Photodiagnosis and Photodynamic Therapy (2020)
2. Efficacy of Antimicrobial Photodynamic Therapy for Elimination of Aggregatibacter Actinomycetemcomitans Biofilm on Laser-Lok Titanium Discs, Photodiagnosis and Photodynamic Therapy (2019)
3. Antimicrobial Efficacy of Photodynamic Therapy Using Two Different Light Sources on the Titanium-Adherent Biofilms of Aggregatibacter Actinomycetemcomitans: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2019)
4. Comparison of Bacterial Disinfection Efficacy Using Blue and Red Lights on Dental Implants Contaminated With Aggregatibacter Actinomycetemcomitans, Photodiagnosis and Photodynamic Therapy (2022)
5. Bactericidal Effect of Erbium-Doped Yttrium Aluminum Garnet Laser and Photodynamic Therapy on Aggregatibacter Actinomycetemcomitans Biofilm on Implant Surface, International Journal of Oral and Maxillofacial Implants (2016)
6. Microstructural Evaluation of Contaminated Implant Surface Treated by Laser, Photodynamic Therapy, and Chlorhexidine 2%, International Journal of Oral and Maxillofacial Implants (2018)
7. The Effect of Apdt With Natural and Chemical Photosensitizers on Reduction of Bacteria Around Orthodontic Brackets, Lasers in Medical Science (2025)
8. Exploring Different Photosensitizers to Optimize Elimination of Planktonic and Biofilm Forms of Enterococcus Faecalis From Infected Root Canal During Antimicrobial Photodynamic Therapy, Photodiagnosis and Photodynamic Therapy (2018)
Experts (# of related papers)
View all Related Experts
Abbas Bahador (33)
Maryam Pourhajibagher (29)
Other Related Docs
9. Ex Vivo Assessment of Synergic Effect of Chlorhexidine for Enhancing Antimicrobial Photodynamic Therapy Efficiency on Expression Patterns of Biofilm-Associated Genes of Enterococcus Faecalis, Photodiagnosis and Photodynamic Therapy (2018)
10. Effects of Sub-Lethal Dose of Antimicrobial Photodynamic Therapy on Major Virulence Traits of Streptococcus Mutans, Photodiagnosis and Photodynamic Therapy (2020)
11. Antimicrobial Photodynamic Therapy Assessment of Three Indocyanine Green-Loaded Metal-Organic Frameworks Against Enterococcus Faecalis, Photodiagnosis and Photodynamic Therapy (2018)
12. Reduction of Streptococcus Salivarius by Chlorellamediated Antimicrobial Photodynamic Therapy, Journal of Lasers in Medical Sciences (2024)
13. Enhanced Photodynamic Suppression of Enterococcus Faecalis Using Curcumin-Loaded Zeolite, Journal of Cluster Science (2022)
14. Comparative Effects of the Conventional, Ultrasonic, and Laser-Activated Irrigation on Penetration Depth of Three Photosensitizers in the Root Canal System, Photodiagnosis and Photodynamic Therapy (2024)
15. Antimicrobial Action of Photoactivated C-Phycocyanin Against Enterococcus Faecalis Biofilms: Attenuation of Quorum-Sensing System, Photodiagnosis and Photodynamic Therapy (2019)
16. The Effect of Sublethal Photodynamic Therapy on the Expression of Enterococcal Surface Protein (Esp) Encoding Gene in Enterococcus Faecalis: Quantitative Real-Time Pcr Assessment, Photodiagnosis and Photodynamic Therapy (2018)
17. Biomaterials and Biological Parameters for Fixed-Prosthetic Implant-Supported Restorations: A Review Study, Advances in Materials Science and Engineering (2022)
18. The Effect of Antimicrobial Photodynamic Therapy on the Expression of Novel Methicillin Resistance Markers Determined Using Cdna-Aflp Approach in Staphylococcus Aureus, Photodiagnosis and Photodynamic Therapy (2017)
19. Antimicrobial Photodynamic Therapy for Managing the Peri-Implant Mucositis and Peri-Implantitis: A Systematic Review of Randomized Clinical Trials, Photodiagnosis and Photodynamic Therapy (2024)
20. In Vitro Comparative Evaluation of the Efficacy of Antimicrobial Photodynamic Therapy, Chlorhexidine, Sodium Fluoride, and Hydrogen Peroxide for Acrylic Resin Disinfection, Journal of Lasers in Medical Sciences (2023)
21. The Evaluation of Cultivable Microbiota Profile in Patients With Secondary Endodontic Infection Before and After Photo-Activated Disinfection, Photodiagnosis and Photodynamic Therapy (2017)
22. The Effect of Antimicrobial Photodynamic Therapy Using Different Concentrations of Phycocyanin Against Streptococcus Mutans, Photodiagnosis and Photodynamic Therapy (2024)
23. Modulation of Virulence in Enterococcus Faecalis Cells Surviving Antimicrobial Photodynamic Inactivation With Reduced Graphene Oxide-Curcumin: An Ex Vivo Biofilm Model, Photodiagnosis and Photodynamic Therapy (2020)
24. Evaluation of Adding Nanosized Natural Zeolite to Photodynamic Therapy Against P. Gingivalis Biofilm on Titanium Disks, Photodiagnosis and Photodynamic Therapy (2021)
25. Effect of Various Laser Wavelengths on Temperature Changes During Periimplantitis Treatment: An in Vitro Study, Implant Dentistry (2018)
26. Antimicrobial Photodynamic Therapy Using Diode Laser Activated Indocyanine Green As an Adjunct in the Treatment of Chronic Periodontitis: A Randomized Clinical Trial, Photodiagnosis and Photodynamic Therapy (2016)
27. Comparison of Different Modes of Photo-Activated Disinfection Against Porphyromonas Gingivalis: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2020)
28. Sonodynamic Excitation of Nanomicelle Curcumin for Eradication of Streptococcus Mutans Under Sonodynamic Antimicrobial Chemotherapy: Enhanced Anti-Caries Activity of Nanomicelle Curcumin, Photodiagnosis and Photodynamic Therapy (2020)
29. Effect of Ultrasonic Activation on the Efficacy of Antimicrobial Photodynamic Therapy: Evaluation of Penetration Depth of Photosensitizer and Elimination of Enterococcus Faecalis Biofilms, Photodiagnosis and Photodynamic Therapy (2019)
30. Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy, Photodiagnosis and Photodynamic Therapy (2017)
31. The Efficacy of Photodynamic and Photothermal Therapy on Biofilm Formation of Streptococcus Mutans: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2017)
32. Photo-Activated Disinfection Based on Indocyanine Green Against Cell Viability and Biofilm Formation of Porphyromonas Gingivalis, Photodiagnosis and Photodynamic Therapy (2017)
33. Photodisinfection Effects of Silver Sulfadiazine Nanoliposomes Doped-Curcumin on Acinetobacter Baumannii: A Mouse Model, Nanomedicine (2020)
34. Photo-Sonodynamic Antimicrobial Chemotherapy Via Chitosan Nanoparticles-Indocyanine Green Against Polymicrobial Periopathogenic Biofilms: Ex Vivo Study on Dental Implants, Photodiagnosis and Photodynamic Therapy (2020)
35. Comparison of the Effects of Er, Cr: Ysgg Laser and Super-Saturated Citric Acid on the Debridement of Contaminated Implant Surfaces, Journal of Lasers in Medical Sciences (2018)
36. Inactivation of Aggregatibacter Actinomycetemcomitans by Two Different Modalities of Photodynamic Therapy Using Toluidine Blue O or Radachlorin As Photosensitizers: An in Vitro Study, Lasers in Medical Science (2015)
37. An Ex Vivo Evaluation of Physico-Mechanical and Anti-Biofilm Properties of Resin-Modified Glass Ionomer Containing Ultrasound Waves-Activated Nanoparticles Against Streptococcus Mutans Biofilm Around Orthodontic Bands, Photodiagnosis and Photodynamic Therapy (2022)
38. An in Vivo Evaluation of Microbial Diversity Before and After the Photo-Activated Disinfection in Primary Endodontic Infections: Traditional Phenotypic and Molecular Approaches, Photodiagnosis and Photodynamic Therapy (2018)
39. Exploring Photoactivated Disinfection-Induced Bystander Effects on Microbial Biofilms of Aggregatibacter Actinomycetemcomitans, Infectious Disorders - Drug Targets (2021)
40. The Anti-Biofilm Activity of Nanometric Zinc Doped Bioactive Glass Against Putative Periodontal Pathogens: An in Vitro Study, Biomedical Glasses (2020)
41. Enhancing Antimicrobial Efficacy Against Pseudomonas Aeruginosa Biofilm Through Carbon Dot-Mediated Photodynamic Inactivation, AMB Express (2024)
42. Clinical Approach of High Technology Techniques for Control and Elimination of Endodontic Microbiota, Journal of Lasers in Medical Sciences (2015)
43. Effect of Antimicrobial Photodynamic Therapy With Different Photosensitizers and Adhesion Protocol on the Bond Strength of Resin Composite to Sound Dentin, Clinical Oral Investigations (2022)
44. Antibacterial Potential of Riboflavin Mediated Blue Diode Laser Photodynamic Inactivation Against Enterococcus Faecalis: A Laboratory Investigation, Photodiagnosis and Photodynamic Therapy (2023)
45. Diagnostic Accuracy of Multiplex Real-Time Pcr Approaches Compared With Cultivation -Based Detection Methods: Monitoring the Endopathogenic Microbiota Pre and Post Photo-Activated Disinfection, Photodiagnosis and Photodynamic Therapy (2018)
46. The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture on Enterococcus Faecalis: The Influence of Different Light Sources, Applied Sciences (Switzerland) (2020)
47. The Potential Application of Natural Photosensitizers Used in Antimicrobial Photodynamic Therapy Against Oral Infections, Pharmaceuticals (2022)
48. Carnosine-Graphene Oxide Conjugates Decorated With Hydroxyapatite As Promising Nanocarrier for Icg Loading With Enhanced Antibacterial Effects in Photodynamic Therapy Against Streptococcus Mutans, Journal of Photochemistry and Photobiology B: Biology (2018)
49. The Influence of Different Mode of Power Density During Antimicrobial Photodynamic Therapy for Photokilling of Streptococcus Mutans, Photodiagnosis and Photodynamic Therapy (2023)
50. The Effect of Indocyanine Green Loaded on a Novel Nano-Graphene Oxide for High Performance of Photodynamic Therapy Against Enterococcus Faecalis, Photodiagnosis and Photodynamic Therapy (2017)