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Combined Effects of High Fat Diet and Exercise on Autophagy in White Adipose Tissue of Mice Publisher Pubmed



Daneshyar S1, 2 ; Tavoosidana G3 ; Bahmani M4 ; Basir SS5 ; Delfan M6 ; Laher I7 ; Saeidi A8 ; Granacher U9 ; Zouhal H10, 11
Authors
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Authors Affiliations
  1. 1. Department of Physical Education, Faculty of Humanities, Ayatollah Alozma Boroujerdi University, Lorestan, Iran
  2. 2. Department of Physical Education, Hamedan University of Technology, Hamedan, Iran
  3. 3. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Biochemistry, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
  5. 5. Department of Exercise Physiology, Faculty of Physical Education, University of Guilan, Guilan, Iran
  6. 6. Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran
  7. 7. Department of Anesthesiology, Pharmacology, and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, Canada
  8. 8. Department of Physical Education and Sport Sciences, Faculty of Humanities and Social Sciences, University of Kurdistan, Kurdistan, Sanandaj, Iran
  9. 9. Department of Sport and Sport Science, Exercise and Human Movement Science, University of Freiburg, Germany
  10. 10. Univ Rennes, M2S (Laboratoire Mouvement, Sport, Sante) - EA 1274, Rennes, F-35000, France
  11. 11. Institut International des Sciences du Sport (2I2S), Irodouer, 35850, France

Source: Life Sciences Published:2023


Abstract

Aim: The effects of nutrition and exercise on autophagy are not well studied. This study aimed to investigate the combined effects of high-fat diets (HFD) and exercise training (ET) on autophagy in white adipose tissue of mice. Materials and methods: Male C57BL/6 mice were assigned into four groups of 7 mice per group: (1) Control, (2) high-fat diet-induced obesity (HFD-Ob), (3) exercise training (ET), and (4) high-fat diet with exercise training (HFD-ET). The HFD-Ob group was fed a high-fat diet for 14 weeks, while the ET group continuously ran on a treadmill for five sessions per week for seven weeks, and the HFD-ET group had both HFD and exercise training. qReal-time–PCR and western blot were used to measure the mRNA and protein levels of autophagy markers in white adipose tissue. Results: Mice from the HFD group showed higher levels in autophagy-related gene5 (ATG5, p = 0.04), ATG7 (p = 0.002), cathepsin B (CTSB, p = 0.0004), LC3-II (p = 0.03) than control. Mice in the ET group displayed higher levels of genes for ATG7 (p = 0.0003), microtubule-associated protein1-light chain 3 (LC3, p = 0.05), lysosome-associated membrane protein 2 (LAMP2, p = 0.04) and cathepsin L (CTSL, p = 0.03) than control. Mice from the HFD-ET group had higher levels of genes for ATG7 (p = 0.05) and CTSL (p = 0.043) and lower levels of genes for CTSB (p = 0.045) compared to the HFD group and lower levels of LAMP2 (p = 0.02) compared to the ET group. Conclusion: There were increases in autophagosome formation in the white adipose tissue from mice in the HFD and ET groups. A combination of HFD and ET enhances autophagosome formation and modulates lysosomal degradation in white adipose tissue. © 2022
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